Veterinary Laboratories Division, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, United Arab Emirates.
Al Wathba Stables, Abu Dhabi, United Arab Emirates.
Vector Borne Zoonotic Dis. 2020 Jun;20(6):412-417. doi: 10.1089/vbz.2019.2537. Epub 2020 Feb 20.
This study was conducted to evaluate the use of Brucellergene skin test (BST) for the diagnosis of Brucellosis in camels () in comparison with Rose Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 68 apparently healthy adult dromedary camels of either gender from three different geographical locations of Abu Dhabi Emirate, United Arab Emirates (UAE), were included in the study. The skin test was applied on two shaved areas at the middle of the neck: one for the test and the other area was injected with normal saline as a control. Reading was done 72 h postinjection. Results were subjected to Bayesian analysis to assess the test performances in camels. The model estimated the following sensitivity and specificity median values: BST: Se = 70.72%, Sp = 98.82%; RBT: Se = 93.27%, Sp = 97.79%; and c-ELISA: Se = 94.78%, Sp = 98.48%. As the BST investigated in this study proved to be a highly specific test, we propose using it as a confirmatory test in camels particularly when the serological tests give doubtful results on individual animals.
本研究旨在评估布鲁氏菌素皮试（BST）在诊断骆驼布鲁氏菌病方面的应用，将其与玫瑰红平板试验（RBT）和竞争酶联免疫吸附试验（c-ELISA）进行比较。从阿联酋阿布扎比酋长国的三个不同地理位置选择了 68 匹性别不同的明显健康成年单峰骆驼参与研究。在颈部中间的两个剃毛区域进行皮试：一个用于测试，另一个区域注射生理盐水作为对照。注射后 72 小时进行读数。结果进行贝叶斯分析，以评估该试验在骆驼中的表现。该模型估计了以下灵敏度和特异性中位数：BST：Se=70.72%，Sp=98.82%；RBT：Se=93.27%，Sp=97.79%；c-ELISA：Se=94.78%，Sp=98.48%。鉴于本研究中调查的 BST 被证明是一种高度特异性的试验，我们建议将其作为骆驼的确认性试验，特别是当血清学试验对个体动物产生可疑结果时。
