Key Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, People's Republic of China.
Analyst. 2020 Apr 7;145(7):2725-2730. doi: 10.1039/c9an01809c. Epub 2020 Feb 20.
AlGaN/GaN high electron mobility transistor (HEMT) biosensors have attracted attention due to their high sensitivity, stability, and fast response characteristics. Some related studies have been explored but a Debye screening problem exists in physiological solutions hindering the detection of bio-macromolecules. Herein, a novel fast analytical platform for electronic enzyme-linked immunosorbent assay (e-ELISA) is proposed based on AlGaN/GaN HEMT with magnetic beads (MBs); MB-based e-ELISA decouples the modified area from the sensing surface to simplify the assay. Combining the advantages of e-ELISA and MBs, the resulting analytical platform presents a sensing capability beyond the Debye-screening limit and a novel ability to be reused. This platform offers a fast response toward prostate specific antigen (PSA) and the lowest concentration of detection is 1 fg mL. Compared with conventional AlGaN/GaN HEMT biosensors, it shows higher sensitivity (3.73 μA dec) in a linear range (1 fg mL to 1 pg mL), which is within the constraints of emergency care applications. The platform's high sensitivity and fast repeatability endow it with great potential for early and rapid diagnosis.
氮化铝镓/氮化镓高电子迁移率晶体管（HEMT）生物传感器因其高灵敏度、稳定性和快速响应特性而受到关注。已经探索了一些相关的研究，但在生理溶液中存在德拜屏蔽问题，阻碍了生物大分子的检测。在此，提出了一种基于氮化铝镓/氮化镓 HEMT 与磁珠（MB）的新型快速电子酶联免疫吸附测定（e-ELISA）分析平台；MB 基 e-ELISA 将修饰区域与传感表面解耦，简化了测定。结合 e-ELISA 和 MB 的优点，所得到的分析平台具有超越德拜屏蔽限制的传感能力和新型可重复使用的能力。该平台对前列腺特异性抗原（PSA）具有快速的响应，检测的最低浓度为 1 fg mL。与传统的氮化铝镓/氮化镓 HEMT 生物传感器相比，在 1 fg mL 至 1 pg mL 的线性范围内，其灵敏度更高（3.73 μA dec），这在紧急护理应用的限制范围内。该平台的高灵敏度和快速可重复性使其具有早期和快速诊断的巨大潜力。
