Bentz J, Nir S, Covell D G
Department of Pharmacy, School of Pharmacy, University of California, San Francisco 94143.
Biophys J. 1988 Sep;54(3):449-62. doi: 10.1016/S0006-3495(88)82978-3.
A simple approximate solution for the mass action kinetics of small particles (viruses or vesicles) binding to large particles (cells) and their subsequent fusion has been derived. The solution is evaluated in terms of the measurable fluorescence changes expected when the virus or vesicles are labeled with fluorescent probes, which are diluted into the cellular membrane by fusion. Comparison with numerical integrations shows that the approximate solution is extremely accurate. Analytic simplifications for a variety of special cases of this general problem are also shown.
已推导出一个关于小颗粒(病毒或囊泡)与大颗粒(细胞)结合及其后续融合的质量作用动力学的简单近似解。该解是根据当病毒或囊泡用荧光探针标记时预期的可测量荧光变化来评估的,这些荧光探针通过融合被稀释到细胞膜中。与数值积分的比较表明,该近似解极其精确。还展示了针对这个一般问题的各种特殊情况的解析简化。