Janes S M, Dalickas G A, Eaton W A, Hochstrasser R M
Department of Chemistry, University of Pennsylvania, Philadelphia 19104-6323.
Biophys J. 1988 Sep;54(3):545-9. doi: 10.1016/S0006-3495(88)82987-4.
The optical transient absorption spectra at 30 ps and 6.5 ns after photolysis are compared for both carboxy hemoglobin (HbCO) and carboxy myoglobin (MbCO). Both 355- and 532-nm excitation pulses were used. In all cases the shapes of the optical difference spectra thus generated are stationary over the complete time-scale studied. The photolysis spectra for MbCO are not significantly different from the equilibrium difference spectra generated on the same picosecond spectrometer when measured to an accuracy of +/- 0.5 nm. In addition, spectral parameters for delegated HbCO generated on the same spectrometer but detected by two different techniques, either by a Vidicon detector or point by point with photomultiplier tubes, are reported; the results are different from some of the previously reported picosecond experiments.
对光解后30皮秒和6.5纳秒时的羧基血红蛋白（HbCO）和羧基肌红蛋白（MbCO）的光学瞬态吸收光谱进行了比较。使用了355纳米和532纳米的激发脉冲。在所有情况下，由此产生的光学差光谱的形状在整个研究的时间尺度上是稳定的。当测量精度达到±0.5纳米时，MbCO的光解光谱与在同一皮秒光谱仪上产生的平衡差光谱没有显著差异。此外，还报告了在同一光谱仪上产生但通过两种不同技术检测的委托HbCO的光谱参数，这两种技术分别是使用光导摄像管探测器或用光电倍增管逐点检测；结果与一些先前报道的皮秒实验不同。
