Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan. Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Biofabrication. 2020 Mar 13;12(2):025027. doi: 10.1088/1758-5090/ab782b.
Extracellular matrices (ECMs) are dynamically altered and remodeled during tissue development. How the dynamic remodeling of ECM affects stem cell functions remains poorly understood due to the difficulty of obtaining biomimetic ECMs. In this study, stepwise osteogenesis-mimicking ECM-deposited hybrid meshes were prepared by culturing human mesenchymal stem cells (hMSCs) in poly (lactic-co-glycolic acid) (PLGA)-collagen hybrid meshes and controlling the stages of the osteogenesis of hMSCs. Three types of hybrid mesh mimicking the ECMs that were secreted from stem cell stage of hMSCs (SC-ECM), early stage (EO-ECM) and late stage (LO-ECM) osteogenesis of hMSCs were prepared. The stepwise osteogenesis-mimicking ECM deposited PLGA-collagen hybrid meshes showed different ECM compositions associated with the stage of osteogenesis. Their effects on the osteogenic differentiation of hMSCs differed. EO-ECM scaffold increased and LO-ECM scaffold moderately promoted the osteogenic differentiation of hMSCs. However, SC-ECM scaffold inhibited the osteogenic differentiation of hMSCs. The novel PLGA-collagen-ECM hybrid meshes will provide useful tools for stem cell culture and tissue engineering.
细胞外基质（ECM）在组织发育过程中会发生动态改变和重塑。由于难以获得仿生 ECM，动态重塑 ECM 如何影响干细胞功能仍知之甚少。在这项研究中，通过在聚（乳酸-共-乙醇酸）（PLGA）-胶原蛋白杂化网中培养人骨髓间充质干细胞（hMSCs）并控制 hMSCs 成骨的阶段，制备了逐步模仿成骨的 ECM 沉积杂化网。模拟 hMSCs 干细胞阶段（SC-ECM）、早期（EO-ECM）和成骨晚期（LO-ECM）分泌的 ECM 的三种类型的杂化网被制备出来。具有逐步模仿成骨的 ECM 沉积的 PLGA-胶原蛋白杂化网显示出与成骨阶段相关的不同 ECM 组成。它们对 hMSCs 成骨分化的影响也不同。EO-ECM 支架增加，LO-ECM 支架适度促进 hMSCs 的成骨分化。然而，SC-ECM 支架抑制 hMSCs 的成骨分化。新型 PLGA-胶原蛋白-ECM 杂化网将为干细胞培养和组织工程提供有用的工具。
