Institute of Virology, University of Cologne, Faculty of Medicine and University Hospital of Cologne, 50935 Cologne, Germany.
National Reference Centre for Poliomyelitis and Enteroviruses, Robert Koch Institute, 13353 Berlin, Germany.
Viruses. 2020 Feb 16;12(2):221. doi: 10.3390/v12020221.
In the post-polio eradication era, increasing attention is given to non-polio enteroviruses. Most of the data about enteroviruses in sub-Saharan Africa are related to acute flaccid paralysis surveillance and target the pediatric population. This study aimed to investigate the presence of enterovirus in PLHIV (people living with HIV) and HIV-negative individuals in Ghana. Stool samples from HIV-positive individuals ( = 250) and healthy blood donors ( = 102) attending the Komfo Anokye Teaching Hospital in Kumasi, Ghana, were screened by real-time PCR for enterovirus. Molecular typing of the VP1 region was performed. Enterovirus-positive samples were tested for norovirus, adenovirus, rotavirus, sapovirus, and cosaviruses. Twenty-six out of 250 HIV-positive subjects (10.4%) and 14 out of 102 HIV-negative individuals (13.7%) were detected enterovirus-positive, not showing a significant different infection rate between the two groups. HIV-negative individuals were infected with strains only. HIV-positive participants were detected positive for species , , and . Co-infections with other viral enteric pathogens were almost exclusively detected among HIV-positive participants. Overall, the present study provides the first data about enteroviruses within HIV-positive and HIV-negative adults living in Ghana.
在后脊髓灰质炎消除时代，人们越来越关注非脊髓灰质炎肠道病毒。撒哈拉以南非洲的大多数肠道病毒数据都与急性弛缓性麻痹监测有关，并且针对儿科人群。本研究旨在调查加纳艾滋病毒感染者（PLHIV）和艾滋病毒阴性个体中肠道病毒的存在情况。通过实时 PCR 对加纳库马西 Komfo Anokye 教学医院就诊的 HIV 阳性个体（= 250）和健康献血者（= 102）的粪便样本进行肠道病毒筛查。对 VP1 区进行分子分型。对肠道病毒阳性样本进行诺如病毒、腺病毒、轮状病毒、星状病毒和冠状病毒检测。在 250 名 HIV 阳性个体中有 26 名（10.4%）和 102 名 HIV 阴性个体中有 14 名（13.7%）检测到肠道病毒阳性，两组之间的感染率没有显著差异。HIV 阴性个体仅感染 株。HIV 阳性参与者检测出 、 和 种。其他肠道病毒病原体的合并感染几乎仅在 HIV 阳性参与者中检测到。总体而言，本研究提供了加纳 HIV 阳性和 HIV 阴性成年人中肠道病毒的首批数据。
