• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

诱导多能干细胞衍生的细胞外囊泡促进急性肾损伤模型中的肾保护作用。

Extracellular Vesicles Derived from Induced Pluripotent Stem Cells Promote Renoprotection in Acute Kidney Injury Model.

机构信息

Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil.

National Institute of Science and Technology for Regenerative Medicine-REGENERA, Federal University of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil.

出版信息

Cells. 2020 Feb 17;9(2):453. doi: 10.3390/cells9020453.

DOI:10.3390/cells9020453
PMID:32079274
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7072760/
Abstract

Induced pluripotent stem cells (iPSC) have been the focus of several studies due to their wide range of application, including in cellular therapy. The use of iPSC in regenerative medicine is limited by their tumorigenic potential. Extracellular vesicles (EV) derived from stem cells have been shown to support renal recovery after injury. However, no investigation has explored the potential of iPSC-EV in the treatment of kidney diseases. To evaluate this potential, we submitted renal tubule cells to hypoxia-reoxygenation injury, and we analyzed cell death rate and changes in functional mitochondria mass. An in vivo model of ischemia-reperfusion injury was used to evaluate morphological and functional alterations. Gene array profile was applied to investigate the mechanism involved in iPSC-EV effects. In addition, EV derived from adipose mesenchymal cells (ASC-EV) were also used to compare the potential of iPSC-EV in support of tissue recovery. The results showed that iPSC-EV were capable of reducing cell death and inflammatory response with similar efficacy than ASC-EV. Moreover, iPSC-EV protected functional mitochondria and regulated several genes associated with oxidative stress. Taken together, these results show that iPSC can be an alternative source of EV in the treatment of different aspects of kidney disease.

摘要

诱导多能干细胞（iPSC）因其广泛的应用而成为了多项研究的焦点，包括细胞疗法。iPSC 在再生医学中的应用受到其致瘤潜能的限制。已经有研究表明，来源于干细胞的细胞外囊泡（EV）可以支持损伤后的肾脏恢复。然而，目前还没有研究探索 iPSC-EV 在治疗肾脏疾病方面的潜力。为了评估这种潜力，我们将肾小管细胞置于缺氧-复氧损伤中，并分析细胞死亡率和功能线粒体质量的变化。使用缺血再灌注损伤的体内模型来评估形态和功能的改变。基因芯片谱用于研究 iPSC-EV 作用涉及的机制。此外，还使用了来源于脂肪间充质细胞的 EV（ASC-EV）来比较 iPSC-EV 支持组织恢复的潜力。结果表明，iPSC-EV 能够以与 ASC-EV 相似的效果降低细胞死亡和炎症反应。此外，iPSC-EV 还保护了功能线粒体并调节了与氧化应激相关的几个基因。总之，这些结果表明，iPSC 可以作为 EV 的替代来源，用于治疗肾脏疾病的不同方面。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/38eb789529cb/cells-09-00453-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/581f7ebc8682/cells-09-00453-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/456051a8b33e/cells-09-00453-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/389fcaf2533f/cells-09-00453-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/95d00ab09202/cells-09-00453-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/d8389b05a46b/cells-09-00453-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/38eb789529cb/cells-09-00453-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/581f7ebc8682/cells-09-00453-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/456051a8b33e/cells-09-00453-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/389fcaf2533f/cells-09-00453-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/95d00ab09202/cells-09-00453-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/d8389b05a46b/cells-09-00453-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5774/7072760/38eb789529cb/cells-09-00453-g006.jpg

相似文献

1
Extracellular Vesicles Derived from Induced Pluripotent Stem Cells Promote Renoprotection in Acute Kidney Injury Model.诱导多能干细胞衍生的细胞外囊泡促进急性肾损伤模型中的肾保护作用。
Cells. 2020 Feb 17;9(2):453. doi: 10.3390/cells9020453.
2
Hypoxic Conditions Promote the Angiogenic Potential of Human Induced Pluripotent Stem Cell-Derived Extracellular Vesicles.缺氧条件促进人诱导多能干细胞衍生的细胞外囊泡的血管生成潜力。
Int J Mol Sci. 2021 Apr 9;22(8):3890. doi: 10.3390/ijms22083890.
3
Induced Pluripotent Stem Cell-Derived Conditioned Medium Attenuates Acute Kidney Injury by Downregulating the Oxidative Stress-Related Pathway in Ischemia-Reperfusion Rats.诱导多能干细胞来源的条件培养基通过下调缺血再灌注大鼠氧化应激相关通路减轻急性肾损伤。
Cell Transplant. 2016;25(3):517-30. doi: 10.3727/096368915X688542. Epub 2015 Jun 30.
4
Adipose-Derived Mesenchymal Stromal Cells Under Hypoxia: Changes in Extracellular Vesicles Secretion and Improvement of Renal Recovery after Ischemic Injury.缺氧条件下脂肪来源的间充质基质细胞：细胞外囊泡分泌的变化及缺血性损伤后肾脏恢复的改善
Cell Physiol Biochem. 2019;52(6):1463-1483. doi: 10.33594/000000102.
5
Induced Pluripotent Stem Cell (iPSC)-Derived Extracellular Vesicles Are Safer and More Effective for Cardiac Repair Than iPSCs.诱导多能干细胞（iPSC）衍生的细胞外囊泡比 iPSC 更安全、更有效地用于心脏修复。
Circ Res. 2018 Jan 19;122(2):296-309. doi: 10.1161/CIRCRESAHA.117.311769. Epub 2017 Nov 8.
6
Isolation of Extracellular Vesicles from Stem Cells.从干细胞中分离细胞外囊泡。
Methods Mol Biol. 2017;1660:389-394. doi: 10.1007/978-1-4939-7253-1_32.
7
Human induced pluripotent stem cell-derived extracellular vesicles reduce hepatic stellate cell activation and liver fibrosis.人诱导多能干细胞衍生的细胞外囊泡可减少肝星状细胞的激活和肝纤维化。
JCI Insight. 2019 Jun 11;5(14):125652. doi: 10.1172/jci.insight.125652.
8
Highly efficient magnetic labelling allows MRI tracking of the homing of stem cell-derived extracellular vesicles following systemic delivery.高效的磁标记允许 MRI 追踪干细胞衍生的细胞外囊泡在全身给药后的归巢。
J Extracell Vesicles. 2021 Jan;10(3):e12054. doi: 10.1002/jev2.12054. Epub 2021 Jan 15.
9
Cell Therapy Using Human Induced Pluripotent Stem Cell-Derived Renal Progenitors Ameliorates Acute Kidney Injury in Mice.使用人诱导多能干细胞衍生的肾祖细胞进行细胞治疗可改善小鼠急性肾损伤。
Stem Cells Transl Med. 2015 Sep;4(9):980-92. doi: 10.5966/sctm.2014-0219. Epub 2015 Jul 21.
10
Induced Pluripotent Stem Cells (iPSC)-derived Mesenchymal Stem Cells (MSCs) Showed Comparable Effects in Repair of Acute Kidney Injury as Compared to Adult MSCs.诱导多能干细胞（iPSC）衍生的间充质干细胞（MSCs）在修复急性肾损伤方面的效果与成体 MSCs 相当。
Urol J. 2020 Mar 16;17(2):204-209. doi: 10.22037/uj.v0i0.5362.

引用本文的文献

1
Human induced pluripotent stem cell derived nanovesicles for cardiomyocyte protection and proliferation.用于心肌细胞保护和增殖的人诱导多能干细胞衍生纳米囊泡
Bioact Mater. 2025 May 2;50:585-602. doi: 10.1016/j.bioactmat.2025.04.017. eCollection 2025 Aug.
2
Effects and Mechanisms of Extracellular Vesicles in Different Models of Acute Kidney Injury.细胞外囊泡在不同急性肾损伤模型中的作用及机制
Stem Cells Int. 2025 Mar 24;2025:1075016. doi: 10.1155/sci/1075016. eCollection 2025.
3
Emerging Frontiers in acute kidney injury: The role of extracellular vesicles.

本文引用的文献

1
Adipose Mesenchymal Cells-Derived EVs Alleviate DOCA-Salt-Induced Hypertension by Promoting Cardio-Renal Protection.脂肪间充质细胞衍生的细胞外囊泡通过促进心肾保护减轻去氧皮质酮盐诱导的高血压。
Mol Ther Methods Clin Dev. 2019 Nov 15;16:63-77. doi: 10.1016/j.omtm.2019.11.002. eCollection 2020 Mar 13.
2
HLSC-Derived Extracellular Vesicles Attenuate Liver Fibrosis and Inflammation in a Murine Model of Non-alcoholic Steatohepatitis.HLSC 衍生的细胞外囊泡可减轻非酒精性脂肪性肝炎小鼠模型的肝纤维化和炎症。
Mol Ther. 2020 Feb 5;28(2):479-489. doi: 10.1016/j.ymthe.2019.10.016. Epub 2019 Oct 28.
3
Dual oxidase 1 and NADPH oxidase 2 exert favorable effects in cervical cancer patients by activating immune response.
急性肾损伤的新兴前沿领域：细胞外囊泡的作用
Bioact Mater. 2025 Feb 18;48:149-170. doi: 10.1016/j.bioactmat.2025.02.018. eCollection 2025 Jun.
4
Exosome Loaded in Microneedle Patch Ameliorates Renal Ischemia-Reperfusion Injury in a Mouse Model.载于微针贴剂中的外泌体可改善小鼠模型中的肾缺血再灌注损伤。
Stem Cells Int. 2025 Jan 15;2025:3106634. doi: 10.1155/sci/3106634. eCollection 2025.
5
Cell therapy in kidney diseases: advancing treatments for renal regeneration.肾脏疾病的细胞疗法：推进肾脏再生治疗
Front Cell Dev Biol. 2024 Dec 11;12:1505601. doi: 10.3389/fcell.2024.1505601. eCollection 2024.
6
Extracellular Vesicles from Human Induced Pluripotent Stem Cells Exhibit a Unique MicroRNA and CircRNA Signature.来自人类诱导多能干细胞的细胞外囊泡呈现出独特的微小RNA和环状RNA特征。
Int J Biol Sci. 2024 Nov 22;20(15):6255-6278. doi: 10.7150/ijbs.100113. eCollection 2024.
7
Extracellular vesicles: opening up a new perspective for the diagnosis and treatment of mitochondrial dysfunction.细胞外囊泡：为线粒体功能障碍的诊断和治疗开辟新视角。
J Nanobiotechnology. 2024 Aug 14;22(1):487. doi: 10.1186/s12951-024-02750-8.
8
Future embracing: exosomes driving a revolutionary approach to the diagnosis and treatment of idiopathic membranous nephropathy.拥抱未来：外泌体推动特发性膜性肾病诊断和治疗的革命性方法。
J Nanobiotechnology. 2024 Aug 8;22(1):472. doi: 10.1186/s12951-024-02633-y.
9
Stem cell-based therapy for fibrotic diseases: mechanisms and pathways.基于干细胞的纤维化疾病治疗：机制与途径。
Stem Cell Res Ther. 2024 Jun 18;15(1):170. doi: 10.1186/s13287-024-03782-5.
10
Comparison of Extracellular Vesicles from Induced Pluripotent Stem Cell-Derived Brain Cells.诱导多能干细胞衍生脑细胞的细胞外囊泡比较。
Int J Mol Sci. 2024 Mar 22;25(7):3575. doi: 10.3390/ijms25073575.
双氧化酶 1 和 NADPH 氧化酶 2 通过激活免疫反应对宫颈癌患者发挥有利作用。
BMC Cancer. 2019 Nov 9;19(1):1078. doi: 10.1186/s12885-019-6202-3.
4
Adipose-Derived Mesenchymal Stromal Cells Under Hypoxia: Changes in Extracellular Vesicles Secretion and Improvement of Renal Recovery after Ischemic Injury.缺氧条件下脂肪来源的间充质基质细胞：细胞外囊泡分泌的变化及缺血性损伤后肾脏恢复的改善
Cell Physiol Biochem. 2019;52(6):1463-1483. doi: 10.33594/000000102.
5
Stem cell-derived extracellular vesicles inhibit and revert fibrosis progression in a mouse model of diabetic nephropathy.干细胞衍生的细胞外囊泡抑制和逆转糖尿病肾病小鼠模型中的纤维化进展。
Sci Rep. 2019 Mar 14;9(1):4468. doi: 10.1038/s41598-019-41100-9.
6
M2 macrophages in kidney disease: biology, therapies, and perspectives.肾脏疾病中的 M2 巨噬细胞：生物学、治疗方法和展望。
Kidney Int. 2019 Apr;95(4):760-773. doi: 10.1016/j.kint.2018.10.041. Epub 2019 Mar 1.
7
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines.细胞外囊泡研究的最低限度信息2018（MISEV2018）：国际细胞外囊泡协会的立场声明及MISEV2014指南的更新
J Extracell Vesicles. 2018 Nov 23;7(1):1535750. doi: 10.1080/20013078.2018.1535750. eCollection 2018.
8
Tumorigenicity-associated characteristics of human iPS cell lines.人诱导多能干细胞系的致瘤性相关特征。
PLoS One. 2018 Oct 4;13(10):e0205022. doi: 10.1371/journal.pone.0205022. eCollection 2018.
9
Generation of patient-specific induced pluripotent stem cell lines from one patient with Jervell and Lange-Nielsen syndrome, one with type 1 long QT syndrome and two healthy relatives.从一名患有杰韦尔和朗格-尼尔森综合征的患者、一名患有1型长QT综合征的患者以及两名健康亲属身上生成患者特异性诱导多能干细胞系。
Stem Cell Res. 2018 Aug;31:174-180. doi: 10.1016/j.scr.2018.07.016. Epub 2018 Jul 25.
10
Mitochondrial mechanisms and therapeutics in ischaemia reperfusion injury.缺血再灌注损伤中的线粒体机制与治疗。
Pediatr Nephrol. 2019 Jul;34(7):1167-1174. doi: 10.1007/s00467-018-3984-5. Epub 2018 Jun 2.