Dunphy J C, Busch K L
Department of Chemistry, Indiana University, Bloomington 47405.
Biomed Environ Mass Spectrom. 1988 Nov;17(5):405-10. doi: 10.1002/bms.1200170510.
Mixtures of enkephalins and bradykinins contained on silica gel thin-layer chromatograms were imaged by secondary ion mass spectrometry. Spatial profiles of the sample distribution in the chromatographic plane were determined using a precision manipulator that moves the sample into and out of the primary beam generated in a liquid metal gallium source. For instance, spatial profiles are conveniently measured by monitoring the protonated molecule [M + H]+ of each compound or, in a selected-sequence monitoring experiment, by monitoring a sequence ion common to the members of each peptide group. The latter experiment is useful for quick location and identification of a class of peptides separated by planar chromatography, including thin-layer chromatography and electrophoresis.
硅胶薄层色谱图上含有的脑啡肽和缓激肽混合物通过二次离子质谱法成像。使用精密操纵器确定色谱平面中样品分布的空间轮廓，该操纵器将样品移入和移出液态金属镓源产生的一次束。例如，通过监测每种化合物的质子化分子[M + H]+，或者在选择序列监测实验中，通过监测每个肽组成员共有的序列离子，可以方便地测量空间轮廓。后一种实验对于快速定位和鉴定通过平面色谱法分离的一类肽很有用，包括薄层色谱法和电泳法。
