Central Laboratory, Nanjing Chest Hospital, Medical School of Southeast University, Nanjing, PR China.
Central Laboratory, Nanjing Brain Hospital, Nanjing Medical University, Nanjing, PR China.
Epigenomics. 2020 Apr;12(8):685-700. doi: 10.2217/epi-2019-0382. Epub 2020 Feb 21.
We aimed to explore the circular RNA (circRNA) profile of small-cell lung cancer (SCLC). Total RNA was extracted from six paired SCLC tumors and adjacent noncancerous tissues. Next-generation sequencing was performed to identify the circRNA expression profile of SCLC. We found that five circRNAs were significantly upregulated and 30 circRNAs were significantly downregulated in the SCLC tissues. We confirmed the five upregulated and four randomly selected downregulated circRNAs using real-time quantitative PCR. Notably, circ-STXBP5L was selectively upregulated in SCLC samples, but undetectable in the normal control tissues. Bioinformatics analysis demonstrated that circ-STXBP5L may participate in SCLC carcinogenesis by regulating numerous cancer-related pathways. This study may provide new insights into the early diagnosis and development of targeted therapies for SCLC.
我们旨在探索小细胞肺癌（SCLC）的环状 RNA（circRNA）图谱。从 6 对 SCLC 肿瘤和相邻非癌组织中提取总 RNA。进行下一代测序以鉴定 SCLC 的 circRNA 表达谱。我们发现 SCLC 组织中有 5 个 circRNA 显著上调，30 个 circRNA 显著下调。我们使用实时定量 PCR 验证了 5 个上调的和 4 个随机选择的下调的 circRNA。值得注意的是，circ-STXBP5L 在 SCLC 样本中选择性地上调，但在正常对照组织中不可检测。生物信息学分析表明，circ-STXBP5L 可能通过调节多种与癌症相关的途径参与 SCLC 的发生。这项研究可能为 SCLC 的早期诊断和靶向治疗的发展提供新的见解。
