Zhang Jiachao, Zhao Jinshan, Jin Hao, Lv Ruirui, Shi Huiwen, De Guozhong, Yang Bo, Sun Zhihong, Zhang Heping
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education P. R. C., Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs P. R. C., Inner Mongolia Agricultural University , Hohhot, P. R. China.
College of Food Science and Engineering, Hainan University , Haikou, P. R. China.
Gut Microbes. 2020 Jul 3;11(4):930-943. doi: 10.1080/19490976.2020.1722054. Epub 2020 Feb 20.
The challenging conditions encountered during long sea voyages increase the risk of health-threatening physiological and psychological stress for sailors compared with land-based workers. However, how the intestinal microbiota responds to a long sea voyage and whether there is a feasible approach for protecting gut health during sea voyage are still unexplored. Here, we designed a 30-d longitudinal study including a placebo group ( = 42) and a probiotic group ( = 40) and used shotgun metagenomic sequencing to explore the impacts of sea voyage on the intestinal microbiome of sailors. By comparing the intestinal microbiome of subjects in the placebo group at baseline (d 0) and at the end of the sea voyage (d 30), we observed an alteration in the intestinal microbiome during the long sea voyage based on the microbial structure; the results revealed an increase in the species and as well as a decrease in some functional features. However, the change in the microbial structure of sailors in the probiotic group between d 0 and d 30 was limited, which indicated a maintenance effect of probiotics on intestinal microbiome homeostasis. At the metagenomic strain level, a generally positive correlation was observed between probiotics and the strains belonging to and , whereas a common negative correlation was observed between probiotics and ; this result revealed the potential mechanism of maintaining intestinal microbiome homeostasis by probiotics. The present study provided a feasible approach for protecting gut health during a long sea voyage.
与陆上工作者相比，长时间海上航行中遇到的具有挑战性的条件增加了水手面临威胁健康的生理和心理压力的风险。然而，肠道微生物群如何应对长时间海上航行以及在海上航行期间是否存在保护肠道健康的可行方法仍未得到探索。在此，我们设计了一项为期30天的纵向研究，包括一个安慰剂组（n = 42）和一个益生菌组（n = 40），并使用鸟枪法宏基因组测序来探索海上航行对水手肠道微生物组的影响。通过比较安慰剂组受试者在基线（第0天）和海上航行结束时（第30天）的肠道微生物组，我们基于微生物结构观察到长时间海上航行期间肠道微生物组发生了改变；结果显示物种[具体物种1]和[具体物种2]增加，以及一些功能特征减少。然而，益生菌组水手在第0天和第30天之间的微生物结构变化有限，这表明益生菌对肠道微生物组稳态具有维持作用。在宏基因组菌株水平上，观察到益生菌与属于[具体菌属1]和[具体菌属2]的菌株之间普遍呈正相关，而益生菌与[具体菌属3]之间普遍呈负相关；这一结果揭示了益生菌维持肠道微生物组稳态的潜在机制。本研究为在长时间海上航行期间保护肠道健康提供了一种可行的方法。
