Reppert S M, Uhl G R
Laboratory of Developmental Chronobiology, Massachusetts General Hospital, Boston 02114.
Brain Res. 1988 Jul 26;456(2):392-6. doi: 10.1016/0006-8993(88)90246-6.
The development of the regulation of vasopressin (prepropressophysin) mRNA in the supraoptic nuclei (SON) of rats was studied using quantitative in situ hybridization. On day 21 of gestation, vasopressin mRNA levels in the SON were increased by osmotic stimulation, with significant positive correlation between vasopressin mRNA levels and plasma osmolality. On day 19 of gestation, vasopressin mRNA levels in the SON were not significantly increased by osmotic stimulation, and no correlation between vasopressin mRNA levels and plasma osmolality was noted. The results suggest that the mechanisms for osmotic regulation of vasopressin mRNA levels develop between days 19 and 21 of gestation, a time course consistent with the initiation of afferent innervation of the SON.
利用定量原位杂交技术研究了大鼠视上核（SON）中血管加压素（前激素原血管加压素）mRNA调控的发育情况。在妊娠第21天，渗透压刺激可使视上核中血管加压素mRNA水平升高，且血管加压素mRNA水平与血浆渗透压之间存在显著正相关。在妊娠第19天，渗透压刺激并未使视上核中血管加压素mRNA水平显著升高，且未观察到血管加压素mRNA水平与血浆渗透压之间存在相关性。结果表明，血管加压素mRNA水平的渗透压调节机制在妊娠第19天至21天之间发育形成，这一时间进程与视上核传入神经支配的起始一致。
