Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, 4072, Australia.
State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian, 116024, China.
Anal Chim Acta. 2020 Mar 22;1103:156-163. doi: 10.1016/j.aca.2019.12.056. Epub 2019 Dec 20.
Gasotransmitter hydrogen sulfide (HS), produced enzymatically in body, has important functions in biological signaling and metabolic processes. An abnormal level of HS expression is associated with different diseases, therefore, development of novel bioanalytical methods for rapid and effective detection of HS in biological conditions is of great importance. In this work, we report the development of a new responsive nanosensor for ratiometric luminescence detection of HS in aqueous solution and live cells. The nanosensor (Ru@FITC-MSN) was prepared by immobilizing a luminescent ruthenium(II) (Ru(II)) complex into a fluorescein isothiocyanate (FITC) conjugated water-dispersible mesoporous silica nanoparticle (MSN), showing dual emission bands at 520 nm (FITC) and 600 nm (Ru complex). The red luminescence of the formed Ru@FITC-MSN was quenched in the presence of Cu. The in-situ generated Ru-Cu@FITC-MSN responded to HS rapidly and selectively, showing a linear ratiometric luminescence change in FITC and Ru(II) channels with the HS concentration (0.5-4 μM). Limit of detection (LoD) and limit of quantification (LoQ) were determined to be 0.36 and 1.21 μM. Followed by investigation of cellular uptake processes, the utility of the nanosensor for ratiometric imaging of HS in live cells and its capability to monitor HS levels in inflammatory breast cancer cells were then demonstrated. This study provides a powerful approach for detection of highly reactive and unstable HS biomolecules in live systems.
气体递质硫化氢（HS）在体内酶促产生，在生物信号转导和代谢过程中具有重要功能。HS 表达水平异常与多种疾病有关，因此，开发用于快速有效检测生物条件下 HS 的新型生物分析方法非常重要。在这项工作中，我们报告了一种用于 HS 在水溶液和活细胞中比率荧光检测的新型响应纳米传感器的开发。纳米传感器（Ru@FITC-MSN）通过将发光钌（II）（Ru（II））配合物固定到荧光素异硫氰酸酯（FITC）缀合的水分散性介孔硅纳米粒子（MSN）中制备，在 520nm（FITC）和 600nm（Ru 配合物）处显示出双发射带。形成的 Ru@FITC-MSN 的红色荧光在存在 Cu 时被猝灭。原位生成的 Ru-Cu@FITC-MSN 对 HS 快速且选择性响应，在 FITC 和 Ru（II）通道中表现出 HS 浓度（0.5-4μM）的线性比率荧光变化。检测限（LoD）和定量限（LoQ）分别确定为 0.36 和 1.21μM。随后研究了细胞摄取过程，然后证明了该纳米传感器在活细胞中对 HS 的比率成像以及监测炎症性乳腺癌细胞中 HS 水平的能力。这项研究为在活系统中检测高反应性和不稳定的 HS 生物分子提供了一种强大的方法。
