Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China; Department of Horticultural Science, North Carolina State University, Raleigh, NC, 27607, USA.
Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China; Ningbo Research Institute, Zhejiang University, Ningbo, 315100, China.
Plant Sci. 2020 Apr;293:110422. doi: 10.1016/j.plantsci.2020.110422. Epub 2020 Jan 24.
The large-scale untargeted proteomic and metabolomic studies were conducted in strawberry (Fragaria × ananassa) cv. Akihime fruit at five developmental stages. We found that some C6 volatiles highly contributed to the enrichment of volatiles at the red stage of strawberry fruit. We found that 12 genes involved in LOX pathway for volatile biosynthesis showed multiple patterns in protein abundance during fruit development and ripening, and 9 out of the 12 genes exhibited a significant increase in their relative expression levels at the red stage of fruit. We also found that the MYB9 gene (FaMYB9) expression level was positively correlated with the content of C6 volatiles (R = 0.989) and with the relative expression level and protein abundance of FaLOX5 at different strawberry fruit developmental stages (R = 0.954). The interaction between FaMYB9 and FaLOX5 was detected by yeast two-hybrid, co-immunoprecipitation (Co-IP), bimolecular fluorescence complementation (BiFC), and immunofluorescence (IF) analyses. Transient silencing of FaMYB9 delayed the fruit development and ripening, resulting in a significant decrease in the contents of C6 volatiles, while overexpression of FaMYB9 increased the fruit development and ripening and the contents of C6 volatiles in Akihime fruit. Therefore, FaMYB9 is positively involved in C6 volatile biosynthesis.
在五个发育阶段对草莓（ Fragaria × ananassa ） cv. Akihime 果实进行了大规模的非靶向蛋白质组学和代谢组学研究。我们发现一些 C6 挥发物对草莓果实红熟阶段挥发物的富集有很大贡献。我们发现，12 个参与挥发性生物合成 LOX 途径的基因在果实发育和成熟过程中表现出蛋白质丰度的多种模式，其中 9 个基因在果实红熟阶段的相对表达水平显著增加。我们还发现 MYB9 基因（ FaMYB9 ）的表达水平与 C6 挥发物的含量呈正相关（ R = 0.989 ），与不同草莓果实发育阶段 FaLOX5 的相对表达水平和蛋白质丰度呈正相关（ R = 0.954 ）。通过酵母双杂交、共免疫沉淀（ Co-IP ）、双分子荧光互补（ BiFC ）和免疫荧光（ IF ）分析检测到 FaMYB9 和 FaLOX5 之间的相互作用。FaMYB9 的瞬时沉默延迟了果实的发育和成熟，导致 C6 挥发物含量显著下降，而过表达 FaMYB9 则加速了果实的发育和成熟以及 Akihime 果实中 C6 挥发物的含量。因此，FaMYB9 正向参与 C6 挥发性生物合成。
