Department of Physics, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA, 01609, USA.
Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA, 01609, USA.
Anal Biochem. 2020 May 1;596:113626. doi: 10.1016/j.ab.2020.113626. Epub 2020 Feb 17.
We have developed new software, Re-track, that will quantify the rates of retraction and protrusion of structures emanating from the central core of a cell, such as neurites or filopodia. Re-Track, uses time-lapse images of cells in TIFF format and calculates the velocity of retraction or protrusion of a selected structure. The software uses a flexible moving boundary and has the ability to correct this boundary throughout analysis. Re-Track is fast, platform independent, and user friendly, and it can be used to follow biological events such as changes in neuronal connections, tip-growing cells such as moss, adaptive migration of cells, and similar behavior in non-biological systems.
我们开发了新的软件 Re-track，可以定量分析从细胞中心核心伸出的结构（如神经突或丝状伪足）的回缩和突出速率。Re-Track 使用 TIFF 格式的细胞延时图像，并计算选定结构的回缩或突出速度。该软件使用灵活的移动边界，并具有在整个分析过程中校正该边界的能力。Re-Track 快速、与平台无关且用户友好，可用于跟踪生物事件，如神经元连接的变化、尖端生长的细胞（如苔藓）、细胞的适应性迁移以及非生物系统中的类似行为。
