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菲洛视野 - 使用深度学习和尖端标记自动分析丝状伪足。

filoVision - using deep learning and tip markers to automate filopodia analysis.

机构信息

Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA.

Graduate Program in Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.

出版信息

J Cell Sci. 2024 Feb 15;137(4). doi: 10.1242/jcs.261274. Epub 2024 Feb 27.

Abstract

Filopodia are slender, actin-filled membrane projections used by various cell types for environment exploration. Analyzing filopodia often involves visualizing them using actin, filopodia tip or membrane markers. Due to the diversity of cell types that extend filopodia, from amoeboid to mammalian, it can be challenging for some to find a reliable filopodia analysis workflow suited for their cell type and preferred visualization method. The lack of an automated workflow capable of analyzing amoeboid filopodia with only a filopodia tip label prompted the development of filoVision. filoVision is an adaptable deep learning platform featuring the tools filoTips and filoSkeleton. filoTips labels filopodia tips and the cytosol using a single tip marker, allowing information extraction without actin or membrane markers. In contrast, filoSkeleton combines tip marker signals with actin labeling for a more comprehensive analysis of filopodia shafts in addition to tip protein analysis. The ZeroCostDL4Mic deep learning framework facilitates accessibility and customization for different datasets and cell types, making filoVision a flexible tool for automated analysis of tip-marked filopodia across various cell types and user data.

摘要

丝状伪足是一种由各种细胞类型用于环境探索的细而充满肌动蛋白的膜突。分析丝状伪足通常需要使用肌动蛋白、丝状伪足尖端或膜标记物来可视化它们。由于伸出丝状伪足的细胞类型多种多样,从变形虫到哺乳动物,对于某些细胞类型和首选的可视化方法,找到一种可靠的适合其的丝状伪足分析工作流程可能具有挑战性。缺乏一种能够仅使用丝状伪足尖端标记自动分析变形虫丝状伪足的工作流程,促使了 filoVision 的开发。filloVision 是一个适应性强的深度学习平台,具有 filoTips 和 filoSkeleton 这两个工具。filloTips 使用单个尖端标记来标记丝状伪足尖端和细胞质,允许在不使用肌动蛋白或膜标记物的情况下提取信息。相比之下,filoSkeleton 将尖端标记信号与肌动蛋白标记相结合,除了尖端蛋白分析之外,还可以更全面地分析丝状伪足轴。ZeroCostDL4Mic 深度学习框架促进了不同数据集和细胞类型的可访问性和定制化,使 filoVision 成为一种灵活的工具,可用于自动分析各种细胞类型和用户数据中的尖端标记丝状伪足。

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