Gustafsson B E, Norin K E
Acta Pathol Microbiol Scand B. 1977 Feb;85B(1):1-8. doi: 10.1111/j.1699-0463.1977.tb01667.x.
Conventional (CONV) rats were fed by stomach tube for five days with either benzylpenicillin, ampicillin, tetracykline, oxitetracykline, neomycin, bacitracin + neomycin, kanamycin or colistin. On the 2nd-3rd day all the animals developed one or several of the following symptoms or characteristics typical for germfree (GF) rats: no coprostanol formation, no stercobilin production, a GF pattern after gel electrophoresis of fecal supernatant and proteolytic activity in the feces. Under the same conditions succinylsulfathiazole or metronidazole had much less pronounced effects than the antibiotics. When clofibrate, acetylsalicylic acid or ferrous sulphate were administered the effects were none or negligible. The GF characteristics persisted for several weeks after the end of the administration of the drugs. In some instances this was the case up to 7 weeks, when the animals were contaminated by anal route with a suspension of the cecum contents from intact CONV animals. On the 2nd day after this treatment the GF characteristics had disappeared.
对常规（CONV）大鼠通过胃管喂食苄青霉素、氨苄青霉素、四环素、土霉素、新霉素、杆菌肽+新霉素、卡那霉素或黏菌素，持续五天。在第2至3天，所有动物出现了以下一种或几种典型的无菌（GF）大鼠症状或特征：无粪甾烷醇形成、无粪胆素产生、粪便上清液凝胶电泳后呈现GF模式以及粪便中有蛋白水解活性。在相同条件下，琥珀酰磺胺噻唑或甲硝唑的作用远不如抗生素明显。当给予氯贝丁酯、乙酰水杨酸或硫酸亚铁时，无作用或作用可忽略不计。药物给药结束后，GF特征持续数周。在某些情况下，长达7周都是如此，此时动物通过肛门途径被来自完整CONV动物的盲肠内容物悬液污染。在这种处理后的第2天，GF特征消失。
