Biocompatibility of Portunus Pelagicus Hydroxyapatite Graft on Human Gingival Fibroblast Cell Culture.

INTRODUCTION

Crab shell (Portunus pelagicus) has the potential to be a source of hydroxyapatite biomaterials that used as bone grafts. Before clinical application, crab shell graft should be tested for its biocompatibility in vitro on human gingival fibroblast.

AIM

This study aimed to determine the biocompatibility of Portunus pelagicus hydroxyapatite graft on human gingival fibroblast cell culture.

METHODS

Human gingival fibroblast cell cultures were divided into control group and treatment group with the addition of hydroxyapatite graft powder from Portunus pelagicus at a concentration of 100 ppm, 50 ppm, and 25 ppm. The synthesis process of hydroxyapatite was conducted by heating at 1000°C then characterizing the compound with SEM-EDX. All samples were incubated in α-MEM medium, then were given MTT material. The cultures on the plate were examined using ELISA reader. The results were analyzed using a Oneway Anova.

RESULTS

The percentage of living cells throughout all treatment group shown results that exceeded the LD50 parameter. The highest percentage of living cells was at 25 ppm concentration group.

CONCLUSION

The hydroxyapatite graft powder from crab shells is biocompatible with human gingival fibroblast cell culture.