Kamadjaja Michael Josef Kridanto, Abraham Janery Fidelia, Laksono Harry
Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Mayjen Prof. Dr. Moestopo 47, Surabaya, Indonesia.
Faculty of Dental Medicine, Universitas Airlangga, Mayjen Prof. Dr. Moestopo 47, Surabaya, Indonesia.
Med Arch. 2019 Dec;73(6):378-381. doi: 10.5455/medarh.2019.73.378-381.
Crab shell (Portunus pelagicus) has the potential to be a source of hydroxyapatite biomaterials that used as bone grafts. Before clinical application, crab shell graft should be tested for its biocompatibility in vitro on human gingival fibroblast.
This study aimed to determine the biocompatibility of Portunus pelagicus hydroxyapatite graft on human gingival fibroblast cell culture.
Human gingival fibroblast cell cultures were divided into control group and treatment group with the addition of hydroxyapatite graft powder from Portunus pelagicus at a concentration of 100 ppm, 50 ppm, and 25 ppm. The synthesis process of hydroxyapatite was conducted by heating at 1000°C then characterizing the compound with SEM-EDX. All samples were incubated in α-MEM medium, then were given MTT material. The cultures on the plate were examined using ELISA reader. The results were analyzed using a Oneway Anova.
The percentage of living cells throughout all treatment group shown results that exceeded the LD50 parameter. The highest percentage of living cells was at 25 ppm concentration group.
The hydroxyapatite graft powder from crab shells is biocompatible with human gingival fibroblast cell culture.
蟹壳（远海梭子蟹）有潜力成为用作骨移植的羟基磷灰石生物材料的来源。在临床应用之前，蟹壳移植物应在体外对人牙龈成纤维细胞进行生物相容性测试。
本研究旨在确定远海梭子蟹羟基磷灰石移植物对人牙龈成纤维细胞培养的生物相容性。
将人牙龈成纤维细胞培养物分为对照组和处理组，处理组添加浓度为100 ppm、50 ppm和25 ppm的远海梭子蟹羟基磷灰石移植物粉末。通过在1000°C加热进行羟基磷灰石的合成过程，然后用扫描电子显微镜-能谱仪（SEM-EDX）对该化合物进行表征。所有样品在α-MEM培养基中孵育，然后给予MTT材料。使用酶标仪检查平板上的培养物。结果采用单因素方差分析进行分析。
所有处理组的活细胞百分比显示结果超过半数致死剂量（LD50）参数。活细胞百分比最高的是在25 ppm浓度组。
蟹壳羟基磷灰石移植物粉末与人牙龈成纤维细胞培养物具有生物相容性。
