壳聚糖凝胶与蟹壳（废弃物）来源的羟基磷灰石联合作为骨移植材料通过白细胞介素-1和骨形态发生蛋白-2分析对牙周组织再生的有效性
Effectiveness of Combination of Chitosan Gel and Hydroxyapatite from Crabs Shells () Waste as Bonegraft on Periodontal Network Regeneration through IL-1 and BMP-2 Analysis.
作者信息
Gani Asdar, Yulianty Risfah, Supiaty Supiaty, Rusdy Machirah, Dwipa Asri Gustivanny, Eka Satya Dian, Rahayu Feblina Ayu, Achmad Harun
机构信息
Department of Periodontology, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.
Department of Pharmaceutical Science and Technology, Faculty of Pharmacy, Hasanuudin University, Makassar, Indonesia.
出版信息
Int J Biomater. 2022 Mar 20;2022:1817236. doi: 10.1155/2022/1817236. eCollection 2022.
BACKGROUND
Periodontitis can be treated by regenerating periodontal tissue using a bone graft. Several natural materials such as chitosan and minerals such as hydroxyapatite can be developed to increase periodontal tissue regeneration. Chitosan has a high potential in healing wounds. Hydroxyapatite has excellent properties such as biocompatibility, osteoconductive, osteoinductive, and osteogenesis, making it an ideal material for soft and hard tissue regeneration. Chitosan and hydroxyapatite can be obtained from the shells of crustaceans, such as crabs shells ().
OBJECTIVE
To assess the effectiveness of the combination of chitosan gel and hydroxyapatite powder as a bone graft on periodontal tissue regeneration in experimental animals. Periodontal tissue regeneration was assessed by expressing inflammatory cytokine gene indicators IL-1 and BMP-2.
METHODS
Experimental laboratory research and clinical trials with posttest only control group design. Twenty-seven Wistar rats were divided into three groups. Then the femoral bone defect was made, the positive control group was given placebo gel, the positive control group was given BATAN hydroxyapatite, and the test group was given a combination of chitosan gel and hydroxyapatite crab shells. Wistar rats were sacrificed on days 7, 14, and 21, and the femur bone was then taken for immunohistochemical analysis to determine the levels of IL-1 and BMP-2. The Kolmogorov-Smirnov test, Levene test, and one-way ANOVA analyzed the data.
RESULTS
On days 7, 14, and 21, the expression levels of IL-1 and BMP2 were significantly different between the three groups. The group added with chitosan gel and crab shell HA showed a faster decrease in IL-1 expression than the control group. BMP-2 expression increased in the test group compared to the control group.
CONCLUSION
The combination of chitosan gel and hydroxyapatite inhibited the production of proinflammatory cytokines and increased the production of BMP-2.
背景
牙周炎可通过使用骨移植材料再生牙周组织来治疗。可以开发几种天然材料如壳聚糖以及矿物质如羟基磷灰石来促进牙周组织再生。壳聚糖在伤口愈合方面具有很大潜力。羟基磷灰石具有诸如生物相容性、骨传导性、骨诱导性和成骨等优异特性，使其成为软组织和硬组织再生的理想材料。壳聚糖和羟基磷灰石可从甲壳类动物的外壳中获取，如蟹壳（）。
目的
评估壳聚糖凝胶和羟基磷灰石粉末组合作为骨移植材料对实验动物牙周组织再生的有效性。通过检测炎症细胞因子基因指标白细胞介素 -1（IL -1）和骨形态发生蛋白 -2（BMP -2）来评估牙周组织再生情况。
方法
采用仅设后测对照组设计的实验性实验室研究和临床试验。将27只Wistar大鼠分为三组（此处原文表述有误，应为三组，根据后文判断）。然后制造股骨缺损，阳性对照组给予安慰剂凝胶，阳性对照组给予国家核能机构（BATAN）的羟基磷灰石，试验组给予壳聚糖凝胶和蟹壳羟基磷灰石的组合。在第7天、14天和21天处死Wistar大鼠，然后取出股骨进行免疫组织化学分析以确定IL -1和BMP -2的水平。采用柯尔莫哥洛夫 - 斯米尔诺夫检验、莱文检验和单因素方差分析对数据进行分析。
结果
在第7天、14天和21天，三组之间IL -1和BMP2的表达水平存在显著差异。添加壳聚糖凝胶和蟹壳羟基磷灰石的组IL -1表达下降速度比对照组更快。与对照组相比，试验组中BMP -2表达增加。
结论
壳聚糖凝胶和羟基磷灰石的组合抑制了促炎细胞因子的产生并增加了BMP -2的产生。
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