Kuzucuoğlu Mustafa, Topuz Ruhan Deniz, Altun Eren
Department of Thoracic Surgery, Balıkesir University Health Application and Research Hospital, Balıkesir, Turkey.
Department of Thoracic Surgery, Medicine Faculty of Trakya University, Edirne, Turkey.
Turk Gogus Kalp Damar Cerrahisi Derg. 2019 Jan 1;27(1):73-79. doi: 10.5606/tgkdc.dergisi.2019.16337. eCollection 2019 Jan.
This study aims to evaluate the effect of mitomycin-C applied through different drug administration approaches on the development of granulation tissue in the field of surgery and renal functions in rats which underwent tracheal surgery.
Fifty male adult Sprague Dawley rats (weighing mean 200 g to 300 g) were divided into five groups. An incision was performed between the fifth and sixth cartilage ring of the trachea in all groups under anesthesia and the incision was primarily repaired with a 6/0 monofilament absorbable suture. A single dose of mitomycin-C 0.5 mg was applied in the experimental animals appropriate with their assigned groups as topical, intraperitoneal injection, injection to the wound edges, and through inhalation. No mitomycin-C was administered in one group which was accepted as the control group. Rats were sacrificed four weeks after surgery and their tracheas were excised subsequently. Tracheal tissue samples were histopathologically evaluated in terms of epithelization, fibrosis, amount of fibroblasts, angiogenesis, and inflammatory response. Diameter and wall thickness of the tracheas were measured. Blood urea and creatinine levels were evaluated for nephrotoxicity, and the rats were immunohistochemically examined for glomerular pathology.
Epithelization was statistically significantly decelerated (p<0.01), diameter of the trachea was statistically significantly larger (p<0.05), and wall thickness of the trachea was significantly thicker in the group with topical mitomycin-C application compared to the control group (p<0.01).
Topically applied mitomycin-C following tracheal surgery slows down epithelization and, thus, decreases the development of granulation tissue and maintains a wider diameter of the trachea.
本研究旨在评估通过不同给药途径应用丝裂霉素-C对接受气管手术的大鼠手术区域肉芽组织形成及肾功能的影响。
将50只成年雄性Sprague Dawley大鼠（平均体重200克至300克）分为五组。所有组在麻醉下于气管的第五和第六软骨环之间做切口，并用6/0单丝可吸收缝线进行一期修复。根据分组情况，对实验动物分别采用局部应用、腹腔注射、伤口边缘注射及吸入的方式给予单剂量0.5毫克丝裂霉素-C。有一组不给予丝裂霉素-C作为对照组。术后四周处死大鼠，随后切除其气管。对气管组织样本进行组织病理学评估，观察上皮化、纤维化、成纤维细胞数量、血管生成及炎症反应情况。测量气管的直径和壁厚。评估血尿素和肌酐水平以检测肾毒性，并对大鼠进行肾小球病理的免疫组织化学检查。
与对照组相比，局部应用丝裂霉素-C组的上皮化在统计学上显著减慢（p<0.01），气管直径在统计学上显著增大（p<0.05），气管壁厚显著增厚（p<0.01）。
气管手术后局部应用丝裂霉素-C会减缓上皮化，从而减少肉芽组织形成，并使气管直径保持更宽。
