Rahman Mohammad Shadiqur, Islam S M Majharul, Haque Anamul, Shahjahan Md
Laboratory of Fish Ecophysiology, Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Toxicol Rep. 2020 Feb 7;7:317-323. doi: 10.1016/j.toxrep.2020.02.004. eCollection 2020.
Sumithion, a synthetic organophosphate, is widely used as an agricultural insecticide and for control of tiger bug ( spp.) in larval rearing for aquaculture. An experiment was conducted to examine the effects of sumithion on embryological and larval development of zebrafish . Fertilized egg (n = 100) and larvae (n = 100) were exposed to six concentrations of sumithion (0, 0.1, 0.2, 0.4, 0.8 and 1.6 mg L) in three replicates. LC values for embryos and larvae were calculated by probit analysis. The 24 h LC value of sumithion for embryo was 0.235 (0.079-0.428) mgL. Increasing sumithion concentrations decreased hatching success and increased embryonic mortality. In embryos, sumithion induced several malformations including immature yolk sac, dark yolk sac, yolk sac bud, broken eggshell and notochord, unhatched eggs. Larval LC values at 24, 48 and 72 h of various doses of sumithion exposure were 0.620 (0.436-0.963), 0.475 (0.302-0.801) and 0.341 (0.177-0.617) mgL, respectively. Various physical deformities, including edema, notochord deformity, yolk sac damage, body arcuation, lordosis and black pigmentation on the yolk sac were evident in response to different concentrations of sumithion. The results of the current study indicate that sumithion exerts developmental toxicity to zebrafish embryos and larvae. It is expected that current findings will increase sensitivity about the toxic effect of sumithion in early development, as well the possibility of similar actions induced by other insecticides and pesticides.
杀螟松是一种合成有机磷酸酯，被广泛用作农业杀虫剂，并用于水产养殖幼体培育中防治虎蝽（某些种类）。进行了一项实验以研究杀螟松对斑马鱼胚胎和幼体发育的影响。将100枚受精卵和100尾幼体分成三组重复，暴露于六种浓度的杀螟松（0、0.1、0.2、0.4、0.8和1.6毫克/升）中。通过概率分析计算胚胎和幼体的半数致死浓度（LC）值。杀螟松对胚胎的24小时半数致死浓度为0.235（0.079 - 0.428）毫克/升。杀螟松浓度增加会降低孵化成功率并增加胚胎死亡率。在胚胎中，杀螟松诱导了几种畸形，包括未成熟卵黄囊、深色卵黄囊、卵黄囊芽、破卵壳和脊索、未孵化的卵。不同剂量杀螟松暴露24、48和72小时时幼体的半数致死浓度分别为0.620（0.436 - 0.963）、0.475（0.302 - 0.801）和0.341（0.177 - 0.617）毫克/升。响应不同浓度的杀螟松，出现了各种身体畸形，包括水肿、脊索畸形、卵黄囊损伤、身体弓形弯曲、脊柱前凸和卵黄囊上的黑色色素沉着。当前研究结果表明，杀螟松对斑马鱼胚胎和幼体具有发育毒性。预计当前研究结果将提高对杀螟松在早期发育中毒性作用的敏感性，以及其他杀虫剂和农药诱导类似作用的可能性。
