Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Mossoró, Brazil.
Biopreserv Biobank. 2020 Jun;18(3):165-170. doi: 10.1089/bio.2019.0109. Epub 2020 Feb 21.
The cryobanks of agouti somatic tissues represent a promising tool for the conservation of this species and of those that are phylogenetically related and endangered. For these purposes, one strategy to guarantee the quality of samples after warming would be to choose the appropriate tissue vitrification technique. Therefore, we evaluated the effects of two different techniques, direct vitrification in cryovials (DVC) and solid-surface vitrification (SSV), on the preservation of ear somatic tissues derived from agoutis kept in a scientific center of creation. Noncryopreserved somatic tissues were used as controls. Although SSV reduced the thickness of the dermis and cartilage ( < 0.05), the epidermal thickness of these samples was observed to be similar to controls ( > 0.05). Notably, the number of fibroblasts was not altered with either technique. However, both vitrification methods led to an increase in the number of perinuclear halos, with a particularly strong increase observed in DVC-derived fragments ( < 0.05). Compared with the DVC group, SSV showed a larger number of normal chondrocytes and smaller number of degenerate chondrocytes. Furthermore, the number of empty lacunae in SSV-derived fragments remained similar to controls ( > 0.05). In summary, SSV was found to be a more efficient method for vitrifying agouti somatic tissues compared with DVC. These results are important for the proper formation of agouti somatic banks, an essential step in the study of biological resources in this species.
颊囊组织的低温保存库是保护该物种及其亲缘关系密切且濒危物种的一种很有前途的工具。出于这个目的，为了保证解冻后样本的质量，可以选择合适的组织玻璃化技术。因此，我们评估了两种不同技术的效果，即直接在 cryovials 中进行玻璃化（DVC）和固-液玻璃化（SSV），对保存在一个创造科学中心的颊囊组织的影响。未冷冻的体细胞组织用作对照。尽管 SSV 减少了真皮和软骨的厚度（ < 0.05），但这些样本的表皮厚度与对照相似（ > 0.05）。值得注意的是，两种玻璃化技术都没有改变成纤维细胞的数量。然而，两种玻璃化方法都导致了核周晕圈数量的增加，其中 DVC 衍生的片段增加更为明显（ < 0.05）。与 DVC 组相比，SSV 显示出更多的正常软骨细胞和更少的退化软骨细胞。此外，SSV 衍生的片段中的空陷窝数量与对照相似（ > 0.05）。总的来说，与 DVC 相比，SSV 被发现是一种更有效的玻璃化颊囊体细胞的方法。这些结果对于正确建立颊囊体细胞库是重要的，这是研究该物种生物资源的重要步骤。
