Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid (UFERSA), Mossoro, RN, Brazil.
Laboratory of Animal Germplasm Conservation, UFERSA, Mossoro, RN, Brazil.
Cryo Letters. 2021 Jul-Aug;42(4):210-219.
Somatic tissue banks represent important tools for the conservation of wild mammals, aiming at the immediate maintenance and safeguarding of biological samples. For agouti, Dasyprocta leporina, studies on the formation of these banks are still scarce, especially regarding protocols of the best cryoprotectant solution employed.
To optimize the cryoprotectant solution [ethylene glycol (EG), dimethyl sulfoxide (DMSO), sucrose (SUC)] used for the cryopreservation of agouti somatic tissues.
We treated ear tissues with various cryoprotectant solutions: 3.0 M EG (EG group), 3.0 M EG and 0.25 M SUC (EG-SUC group), 3.0 M DMSO (DMSO group), 3.0 M DMSO and 0.25 M SUC (DMSO-SUC group), 1.5 M EG and 1.5 M DMSO (EG-DMSO group) and 1.5 M EG, 1.5 M DMSO and 0.25 M SUC (EG-DMSO-SUC group). Non-cryopreserved tissues were used as controls. All tissues were analyzed for their ultrastructural and morphometric characteristics by scanning electron microscopy and conventional histology.
EG-DMSO-SUC was found to be the optimal cryoprotectant solution in terms of the evaluated parameters, such as thickness of the dermis and skin, number of perinuclear halos, proliferative potential, number of empty lacunas and degenerated chondrocytes.
Agouti somatic tissue cryopreservation may serve for its conservation and as an experimental model for the development of preservation methods for species of the same genus that are either vulnerable or critically endangered.
体组织库是保护野生动物的重要工具，旨在对生物样本进行即时的维护和保护。对于箭豚，Dasyprocta leporina，关于这些银行的形成的研究仍然很少，特别是关于所使用的最佳冷冻保护剂溶液的协议。
优化用于冷冻保存箭豚体组织的冷冻保护剂溶液[乙二醇（EG），二甲基亚砜（DMSO），蔗糖（SUC）]。
我们用各种冷冻保护剂溶液处理耳组织：3.0 M EG（EG 组），3.0 M EG 和 0.25 M SUC（EG-SUC 组），3.0 M DMSO（DMSO 组），3.0 M DMSO 和 0.25 M SUC（DMSO-SUC 组），1.5 M EG 和 1.5 M DMSO（EG-DMSO 组）和 1.5 M EG，1.5 M DMSO 和 0.25 M SUC（EG-DMSO-SUC 组）。未冷冻保存的组织用作对照。所有组织均通过扫描电子显微镜和常规组织学分析其超微结构和形态特征。
就评估的参数而言，例如真皮和皮肤的厚度，核周晕圈的数量，增殖潜力，空腔的数量和退化的软骨细胞，发现 EG-DMSO-SUC 是最佳的冷冻保护剂溶液。
箭豚体组织的冷冻保存可用于其保存，并可作为同种易危或濒危物种保存方法开发的实验模型。
