Sui MeiXia, Wang ZongWei, Xi Dan, Wang HanHai
College of Biological and Agricultural Engineering, Weifang University, Weifang, China.
Shandong Key Laboratory of Biochemistry and Molecular Biology in Universities, Weifang University, Weifang, China.
Reprod Domest Anim. 2020 May;55(5):613-623. doi: 10.1111/rda.13660. Epub 2020 Mar 15.
MicroRNAs (miRNAs) regulate genes through post-transcriptional regulation by targeting the 3'-UTR of mRNA to downregulate gene expression. Several reports have indicated that miRNA regulation can affect many physiological processes, including immune function, apoptosis, cell proliferation and differentiation, and milk fat metabolism. In this study, miR-142-5P promoted milk fat metabolism, and inhibition of miR-142-5P suppressed milk fat metabolism both in vivo and in vitro. The luciferase and Western blot assays indicated that catenin beta-1 (CTNNB1) is a potential target for miR-142-5P. In addition, CTNNB1 inhibited milk fat metabolism. In summary, miR-142-5P may promote milk fat metabolism by inhibiting CTNNB1 expression.
微小RNA（miRNA）通过靶向mRNA的3'-非翻译区进行转录后调控来调节基因，从而下调基因表达。多项报告表明，miRNA调控可影响许多生理过程，包括免疫功能、细胞凋亡、细胞增殖与分化以及乳脂肪代谢。在本研究中，miR-142-5P促进了乳脂肪代谢，而抑制miR-142-5P在体内和体外均抑制了乳脂肪代谢。荧光素酶和蛋白质免疫印迹分析表明，β-连环蛋白1（CTNNB1）是miR-142-5P的一个潜在靶点。此外，CTNNB1抑制了乳脂肪代谢。总之，miR-142-5P可能通过抑制CTNNB1的表达来促进乳脂肪代谢。
