Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.
Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.
Pharm Biol. 2020 Dec;58(1):184-199. doi: 10.1080/13880209.2020.1717549.
Nephrotoxicity is a renal dysfunction that arises from direct exposure to environmental chemicals or as a side effect of therapeutic drugs. Linn. (Nyctaginaceae), Wall. Ex. Meissn. (Polygonaceae), Gaertn. (Nelumbonaceae) and Buch-Ham. (Capparidaceae) are well-recognized medicinal plants of Indian traditional system of medicine used for kidney disorders. The present investigation was undertaken to develop a chromatographically characterized polyherbal combination and to evaluate its nephroprotective activity. Roots of and , flowers of and stem bark of were extracted by decoction using 70% ethanol. Response surface methodology (RSM) was used for the optimization of extraction parameters. Polyherbal combinations with different doses (150-300 mg/kg) were tested against methotrexate-induced nephrotoxicity in Wistar rats. The optimized extract contained 27% phenols and 15% flavonoids, which showed 75% 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging potential. Based on the retention time of high-performance liquid chromatography (HPLC) analysis, 17 out of 122 constituents were found common in all extracts and combinations. Two combinations showed significantly higher ( ≤ 0.05) DPPH scavenging potential and xanthine oxidase inhibition. The half maximal inhibitory concentration (IC) of the best combination for DPPH scavenging and xanthine oxidase inhibition were 80 and 74 µg/mL, respectively. Treatment of methotrexate-induced nephrotoxic rats with polyherbal combination significantly ( ≤ 0.05) improved the kidney function markers, oxidative stress markers and histological parameters. The developed combination was found to be effective in nephrotoxicity; it can be explored further for the management of drug-induced nephrotoxicity and other chronic kidney diseases.
肾毒性是一种由直接暴露于环境化学物质或治疗药物的副作用引起的肾功能障碍。印度传统医学中使用 Linn.（茜草科）、Wall. Ex. Meissn.（蓼科）、Gaertn.（莲科）和 Buch-Ham.（山柑科）等药用植物来治疗肾脏疾病。本研究旨在开发一种具有特征性色谱的复方草药，并评估其肾保护活性。通过 70%乙醇煎煮法提取 和 的根、 和 的花以及 的茎皮。响应面法（RSM）用于优化提取参数。用不同剂量（150-300mg/kg）的复方草药组合来测试对 methotrexate 诱导的 Wistar 大鼠肾毒性的影响。优化后的提取物含有 27%的酚类化合物和 15%的类黄酮，对 1,1-二苯基-2-苦基肼（DPPH）的清除能力达到 75%。根据高效液相色谱（HPLC）分析的保留时间，在所有提取物和组合中发现了 17 种共有成分。两种组合显示出更高的 DPPH 清除能力和黄嘌呤氧化酶抑制作用（ ≤ 0.05）。最佳组合对 DPPH 清除和黄嘌呤氧化酶抑制的半数最大抑制浓度（IC）分别为 80 和 74μg/mL。用复方草药组合治疗 methotrexate 诱导的肾毒性大鼠可显著改善肾功能标志物、氧化应激标志物和组织学参数（ ≤ 0.05）。该复方草药组合在肾毒性方面具有有效性，可进一步探索其用于管理药物诱导的肾毒性和其他慢性肾病。
