College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Biol Macromol. 2020 May 15;151:384-393. doi: 10.1016/j.ijbiomac.2020.02.197. Epub 2020 Feb 19.
We investigated the effect of lotus seed resistant starch (LRS) on mice fecal microbiota tolerance to bile salt by culturing organisms compared to inulin (INU) glucose (GLU) and waxy corn starch (WAX). Operational taxonomic units (OTUs) and diversity indices in LRS and INU groups were increased in the presence of 0.03% to 0.3% bile salt, while they were decreased in GLU, and OTUs were decreased in WAX. Specifically, LRS promoted proliferation of Lactobacillus, which potentially used bile acid, and inhibited growth of the potentially harmful bacteria Enterococcus and Staphylococcus. Moreover, Lactobacillus was negatively correlated with Salinicoccus and Granulicatella in GLU, LRS and INU groups at 1.5% bile salt. With LRS, amino acid metabolic pathways were increased while pathogens causing certain diseases were decreased. LRS increased the tolerance of mice fecal microbiota to bile salt by promoting the proliferation of bacteria utilizing bile acid and inhibiting the growth of harmful bacteria.
我们研究了莲子抗性淀粉（LRS）对微生物群落的影响，与菊粉（INU）、葡萄糖（GLU）和蜡质玉米淀粉（WAX）相比，LRS 能提高胆汁盐耐受的微生物群落的分类单元（OTUs）和多样性指数。在 0.03%至 0.3%的胆汁盐存在下，LRS 和 INU 组的 OTUs 增加，而 GLU 组则减少，WAX 组的 OTUs 减少。具体而言，LRS 促进了潜在利用胆汁酸的乳酸菌的增殖，同时抑制了潜在有害细菌肠球菌和葡萄球菌的生长。此外，在 1.5%胆汁盐下，GLU、LRS 和 INU 组中，乳酸菌与 Salinicoccus 和 Granulicatella 呈负相关。用 LRS 处理后，氨基酸代谢途径增加，而导致某些疾病的病原体减少。LRS 通过促进利用胆汁酸的细菌的增殖和抑制有害细菌的生长，提高了小鼠粪便微生物群对胆汁盐的耐受性。
