College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Food Chem. 2023 Mar 15;404(Pt A):134599. doi: 10.1016/j.foodchem.2022.134599. Epub 2022 Oct 13.
Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats. LRS inhibited the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group in hyperlipidemic rats. Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats. Furthermore, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group were positively correlated with Dehydro-LCA, isoLCA, TCA, LCA-3-S, TCHO, TG and LDL-C. In summary, LRS improves blood lipid levels by regulating small intestinal flora and accelerating the breakdown of cholesterol into bile acids in the liver.
普通和高脂血症大鼠灌胃莲子抗性淀粉（LRS），并确定四组大鼠的小肠菌群结构和胆汁酸组成，构建不同细菌属、胆汁酸和血脂谱之间的关系网络图，揭示 LRS 降低高脂血症大鼠血脂的微生物机制。LRS 抑制高脂血症大鼠中 Romboutsia、芽孢杆菌、布劳特氏菌、norank_f__Muribaculaceae 和 norank_f__Eubacterium_coprostanoligenes_group 的生长。同时，LRS 促进初级胆汁酸（CA、CDCA、β-MCA）和次级胆汁酸（LCA、UDCA）的产生，并降低高脂血症大鼠中 TCA、Dehydro-LCA、isoLCA、LCA-3-S 和 THDCA 的含量。此外，Blautia、norank_f__Muribaculaceae 和 norank_f__Eubacterium_coprostanoligenes_group 与 Dehydro-LCA、isoLCA、TCA、LCA-3-S、TCHO、TG 和 LDL-C 呈正相关。总之，LRS 通过调节小肠菌群和加速肝脏胆固醇分解为胆汁酸来改善血脂水平。
