Xie Li-Juan, Tan Wei-Juan, Yang Yi-Cong, Tan Yi-Fang, Zhou Ying, Zhou De-Mian, Xiao Shi, Chen Qin-Fang
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Plants (Basel). 2020 Feb 18;9(2):262. doi: 10.3390/plants9020262.
In , LONG-CHAIN ACYL-COA SYNTHETASEs (LACSs) catalyze the synthesis of long-chain acyl-CoAs and function in diverse biological processes. We have recently revealed that LACS2 is primarily involved in the production of polyunsaturated linolenoyl-CoA, essential for the activation of ethylene response transcription factors-mediated hypoxia signaling. Here, we further reported the dual role of LACS2 in the regulation of submergence tolerance by modulating cuticle permeability in Arabidopsis cells. -overexpressors () showed improved tolerance to submergence, with higher accumulation of cuticular wax and cutin in their rosettes. In contrast, knockout of in the mutant resulted in hypersensitivity to submergence with reduced wax crystals and thinner cutin layer. By analyses of plant surface permeability, we observed that the hypoxic sensitivities in the and mutant were physiologically correlated with chlorophyll leaching, water loss rates, ionic leakage, and gas exchange. Thus, our findings suggest the role of LACS2 in plant response to submergence by modulating cuticle permeability in plant cells.
在[具体情境未提及]中，长链脂酰辅酶A合成酶（LACSs）催化长链脂酰辅酶A的合成，并在多种生物过程中发挥作用。我们最近发现，LACS2主要参与多不饱和亚麻酸辅酶A的生成，这对于乙烯反应转录因子介导的缺氧信号激活至关重要。在此，我们进一步报道了LACS2在拟南芥细胞中通过调节角质层通透性对耐淹性调控的双重作用。[LACS2]过表达植株（[具体植株名称未提及]）表现出对淹水的耐受性提高，莲座叶中角质蜡质和角质的积累更多。相反，在[具体突变体名称未提及]突变体中敲除[LACS2]导致对淹水超敏感，蜡质晶体减少且角质层变薄。通过对植物表面通透性的分析，我们观察到[具体植株名称未提及]和[具体突变体名称未提及]突变体中的缺氧敏感性在生理上与叶绿素浸出、失水率、离子渗漏和气体交换相关。因此，我们的研究结果表明LACS2通过调节植物细胞中的角质层通透性在植物对淹水的反应中发挥作用。
