Immunology Division, LCMN, Germans Trias i Pujol University Hospital and Research Institute, FOCIS Center of Excellence-UAB-Barcelona, 08916 Badalona, Spain.
Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Int J Mol Sci. 2020 Feb 18;21(4):1389. doi: 10.3390/ijms21041389.
The current gold-standard diagnostic technique for IgA nephropathy (IgAN), the leading form of primary glomerulonephritis, is renal biopsy. CD89 (the main IgA receptor) is expressed on the surface of monocytes and plays a role in disease pathogenesis. Immunocomplexes formed by sCD89 (soluble form) and Gd-IgA1 are related to disease prognosis. We hypothesize that reduced CD89 surface expression on monocytes may be a marker of disease severity. We aimed to analyze leukocyte subpopulations in peripheral blood and CD89 surface expression on monocytes in a prospective study of 22 patients and 12 healthy subjects (HS). Leukocyte subpopulations and CD89 expression were analyzed by flow cytometry. IgAN patients had a higher percentage of activated and effector memory CD4 and CD8 T lymphocytes, a lower percentage of transitional B lymphocytes and plasmablasts, and a higher percentage of CD56CD16 NK cells and myeloid dendritic cells compared with HS. Correlations between reduced CD89 expression levels on nonclassical monocytes, histological findings of a poor prognosis on renal biopsy and baseline renal function were observed. IgAN patients show a characteristic immunological pattern in peripheral blood. A reduced expression level of CD89 on nonclassical monocytes identifies patients with a worse renal prognosis.
目前，IgA 肾病（IgAN）的金标准诊断技术是肾活检。CD89（主要的 IgA 受体）表达在单核细胞表面，在疾病发病机制中发挥作用。sCD89（可溶性形式）和 Gd-IgA1 形成的免疫复合物与疾病预后有关。我们假设单核细胞表面 CD89 表达减少可能是疾病严重程度的标志。我们旨在通过前瞻性研究分析 22 例患者和 12 例健康对照者（HS）的外周血白细胞亚群和单核细胞表面 CD89 表达。通过流式细胞术分析白细胞亚群和 CD89 表达。与 HS 相比，IgAN 患者的活化和效应记忆 CD4 和 CD8 T 淋巴细胞比例较高，过渡性 B 淋巴细胞和浆母细胞比例较低，CD56CD16 NK 细胞和髓样树突状细胞比例较高。观察到非经典单核细胞 CD89 表达水平降低与肾活检的不良预后组织学发现和基线肾功能之间存在相关性。IgAN 患者在外周血中表现出一种特征性的免疫模式。非经典单核细胞 CD89 表达水平降低可识别出肾功能较差的患者。
