CHU of Québec-Laval University Research Center and Center of Research in Experimental Organogenesis of Laval University/LOEX, Québec, Canada.
Department of Surgery, Faculty of Medicine, Université Laval, Québec, Canada.
Tissue Eng Part C Methods. 2020 Mar;26(3):180-189. doi: 10.1089/ten.TEC.2019.0268.
Innovative therapies combining gene-corrected stem cells and the production of bioengineered tissues to treat epidermolysis bullosa are emerging. However, quantitative tests to measure the adhesion forces between two highly viscoelastic substrates such as those found in bilayered bioengineered skin are needed and are still lacking. The objective of this study was to develop a mechanical test to measure the dermal-epidermal adhesion strength of our bilayered tissue-engineered skin substitute (TES) produced with the self-assembly method. We developed a peel test, which allows the displacement of both skin layers in a T configuration, based on the ASTM International standard. A MATLAB program was written to process and analyze raw data. The experimental setup was tested by measuring the dermal-epidermal adhesion strength in TESs produced with normal or collagen VII-deficient cells. Our peel testing method allowed us to detect the impact of the absence of collagen VII in the dermal-epidermal adhesion strength of TESs and also to examine the progression of the dermal-epidermal adhesion strength in relation to culture time in normal TES. Impact statement This study describes a method for assessing the adhesion strength at the dermal-epidermal junction of individual tissue-engineered skin substitute (TES). An ASTM standardized protocol of peel testing was designed to measure this important mechanical property. Our innovative approach will serve as a quality control in the production, improvement, and application of TESs for the treatment of pathologies affecting the dermal-epidermal adhesion such as epidermolysis bullosa. Data presented contribute to research on the interfaces between biological substrates and provide a reference factor for the characterization of products derived from tissue engineering.
新兴的治疗方法结合了基因修正的干细胞和生物工程组织的生产，以治疗大疱性表皮松解症。然而，需要定量测试来测量双层生物工程皮肤等高度粘弹性基底之间的粘附力，但目前仍缺乏这种测试。本研究的目的是开发一种机械测试来测量我们用自组装法生产的双层组织工程皮肤替代物（TES）的真皮-表皮粘附强度。我们根据 ASTM 国际标准开发了一种剥离测试，允许在 T 型配置中同时移动两层皮肤。编写了一个 MATLAB 程序来处理和分析原始数据。通过测量正常或缺乏胶原 VII 的细胞生产的 TES 的真皮-表皮粘附强度来测试实验设置。我们的剥离测试方法使我们能够检测到胶原 VII 缺乏对 TES 真皮-表皮粘附强度的影响，并且还可以检查正常 TES 中与培养时间相关的真皮-表皮粘附强度的进展。影响描述本研究描述了一种评估个体组织工程皮肤替代物（TES）真皮-表皮交界处粘附强度的方法。设计了一个符合 ASTM 标准的剥离测试协议来测量这个重要的机械性能。我们的创新方法将作为 TES 生产、改进和应用的质量控制，用于治疗影响真皮-表皮粘附的疾病，如大疱性表皮松解症。所提供的数据有助于研究生物基质之间的界面，并为组织工程产品的特性提供参考因素。
