Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russia.
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Mar 15;1141:122014. doi: 10.1016/j.jchromb.2020.122014. Epub 2020 Feb 1.
The aim of this study was to develop a rapid and sensitive immunochromatographic test system for the detection of lincomycin (LIN), which belongs to the lincosamide group of antibiotics and contaminates food products of animal origin. Two formats of immunochromatographic analysis (ICA) based on different approaches of introducing gold nanoparticles (GNPs) as a label were compared. It was demonstrated that an indirect ICA method where GNPs were conjugated with anti-species antibodies allowed the achievement of both instrumental and visual detection limits of LIN almost two orders of magnitude lower than those achieved in the standard direct ICA format. In the optimized conditions, the developed indirect ICA allowed for the detection of LIN within 15 min, with instrumental and visual detection limits of 8 pg/mL and 0.8 ng/mL. The assay showed 40% cross-reactivity to clindamycin (CLIN) as a structural analogue of LIN, with no interaction with antibiotics from other classes. The developed ICA was applied for LIN detection in a panel of food products. No treatment of cow milk was necessary before the analysis. For chicken eggs and honey, a simple procedure of preliminary sample preparation was developed, which fully prevented a matrix influence on the assay results. It was demonstrated that ICA could detect LIN in food products while preserving the same analytical characteristics as in the buffer. The analytical recoveries of LIN in foodstuffs were 93.8-125% with coefficients of variations of 5.3-14.0%.
本研究旨在开发一种用于检测林可霉素（LIN）的快速灵敏免疫层析测试系统，林可霉素属于林可酰胺类抗生素，会污染动物源食品。比较了两种基于引入金纳米颗粒（GNPs）作为标记物的不同方法的免疫层析分析（ICA）格式。结果表明，间接 ICA 方法将 GNPs 与抗物种抗体偶联，可以实现 LIN 的仪器和视觉检测限几乎低两个数量级，优于标准直接 ICA 格式。在优化条件下，开发的间接 ICA 允许在 15 分钟内检测到 LIN，仪器和视觉检测限分别为 8 pg/mL 和 0.8 ng/mL。该测定法对林可霉素的结构类似物克林霉素（CLIN）的交叉反应率为 40%，与其他类别的抗生素无相互作用。开发的 ICA 用于检测食品中的 LIN。在分析之前，无需对牛奶进行处理。对于鸡蛋和蜂蜜，开发了一种简单的样品预处理程序，完全防止了基质对测定结果的影响。结果表明，ICA 可以在不影响分析特性的情况下检测食品中的 LIN。LIN 在食品中的分析回收率为 93.8-125%，变异系数为 5.3-14.0%。
