Sickle-Santanello B J, Farrar W B, DeCenzo J F, Keyhani-Rofagha S, Klein J, Pearl D, Laufman H, O'Toole R V
Department of Surgery, Grant Medical Center, Columbus, Ohio 43215.
Cytometry. 1988 Nov;9(6):594-9. doi: 10.1002/cyto.990090613.
Flow cytometric DNA analysis of paraffin-embedded solid tumors has permitted review of large series of archival tissue in attempts to relate abnormal DNA content to prognosis. Limitations of the technique include: 1) a laborious, time-consuming procedure; 2) variation in technique between laboratories; and 3) lack of an objective method of computing DNA indices. Critical evaluation of our technique has shortened the time involved in dewaxing and rehydration, selectively utilized patient's own normal tissue as the internal standard, proved reproducibility of stored specimens, standardized DNA index computation, and developed a statistical analysis to confirm aneuploidy. These technical improvements and the development of a statistical analysis provide a way to shorten the procedure time and standardize the data generated from flow cytometric DNA analysis so as to improve the quality of retrospective reviews of paraffin-embedded tumors and accelerate the definition of flow cytometry's role as a prognostic indicator.
对石蜡包埋实体瘤进行流式细胞术DNA分析，使得人们能够回顾大量存档组织，以试图将异常DNA含量与预后相关联。该技术的局限性包括：1）操作繁琐、耗时；2）不同实验室之间技术存在差异；3）缺乏计算DNA指数的客观方法。对我们的技术进行严格评估后，缩短了脱蜡和水化所需时间，选择性地将患者自身的正常组织用作内标，证明了储存标本的可重复性，规范了DNA指数计算，并开展了一项统计分析以确认非整倍体。这些技术改进以及统计分析的开展提供了一种缩短程序时间并规范流式细胞术DNA分析所产生数据的方法，从而提高石蜡包埋肿瘤回顾性研究的质量，并加速明确流式细胞术作为预后指标的作用。
