Guzhova I V, Margulis B A
Eksp Onkol. 1988;10(5):31-2.
Cells of rat rhabdomyosarcoma RA-2 selected for high metastatic potential and affinity to lung tissue were (125I)-labelled in the presence of iodogene. The spectrum of 125I-labelled cell surface antigens was investigated by SDS-PAGE. Radionuclide was incorporated by proteins (or glycoproteins) with molecular weight of 30, 36, 47, 57, 78, 90, 102, 124, 180 and 250 kDa. After incubation of cells with 0.05-0.2% triton X-100 solution all the proteins except for those with molecular weight of 47 and 57 kDa were washed off the cellular surface; metastatic potential (MP) of triton-100 treated cells was 10 times as low as that of intact cells.
选择具有高转移潜能且对肺组织有亲和力的大鼠横纹肌肉瘤RA - 2细胞，在碘甘醚存在的情况下用（125I）进行标记。通过SDS - PAGE研究125I标记的细胞表面抗原谱。放射性核素与分子量为30、36、47、57、78、90、102、124、180和250 kDa的蛋白质（或糖蛋白）结合。用0.05 - 0.2% Triton X - 100溶液孵育细胞后，除分子量为47和57 kDa的蛋白质外，所有蛋白质均从细胞表面洗脱；Triton - 100处理细胞的转移潜能（MP）是完整细胞的十分之一。
