Czelejewska Wioleta, Zmijewska Agata, Dziekonski Mariusz, Okrasa Stanislaw
Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Reprod Domest Anim. 2020 May;55(5):604-612. doi: 10.1111/rda.13659. Epub 2020 Mar 15.
In pigs, plasma prolactin concentration markedly changes during the oestrous cycle and the regulation of its secretion is very complex. The contribution of neurokinins in this process has not been sufficiently delineated. The aim of the study was to examine the effects of neurokinin A (NKA) on prolactin synthesis and secretion in cyclic gilts. The expression of NKA precursor (Ppta) and receptor (Tacr2) genes as well as NKA and TACR proteins content in the porcine pituitaries (days 2-3, 9-10, 12-13, 15-16 and 19-20 of the cycle) was determined. Furthermore, the in vitro influence of NKA on the expression of prolactin (Prl), dopamine receptor (D2r), TRH receptor (Trhr) genes and prolactin secretion by the porcine pituitary cells (days 9-10, 15-16 and 19-20 of the cycle) was assessed. The expression of Ppta and Tacr2 as well as NKA and TACR proteins in the pituitary tissue has been changing throughout the oestrous cycle. NKA affected in vitro the expression of studied genes and prolactin secretion depending on the stage of the cycle, dose of NKA and/or duration of the cell incubation. Altogether, the study indicates that NKA is engaged in the modulation of prolactin secretion in the pig during the oestrous cycle.
在猪中，血浆催乳素浓度在发情周期中会发生显著变化，其分泌调节非常复杂。神经激肽在这一过程中的作用尚未得到充分阐明。本研究的目的是检测神经激肽A（NKA）对发情周期母猪催乳素合成和分泌的影响。测定了猪垂体中NKA前体（Ppta）和受体（Tacr2）基因的表达以及NKA和TACR蛋白的含量（在发情周期的第2 - 3天、9 - 10天、12 - 13天、15 - 16天和19 - 20天）。此外，评估了NKA对猪垂体细胞（发情周期的第9 - 10天、15 - 16天和19 - 20天）催乳素（Prl）、多巴胺受体（D2r）、促甲状腺激素释放激素受体（Trhr）基因表达以及催乳素分泌的体外影响。垂体组织中Ppta和Tacr2以及NKA和TACR蛋白的表达在整个发情周期中一直在变化。NKA在体外对所研究基因表达和催乳素分泌的影响取决于发情周期阶段、NKA剂量和/或细胞孵育时间。总之，该研究表明NKA参与了发情周期期间猪催乳素分泌的调节。
