Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
J Physiol Pharmacol. 2020 Apr;71(2). doi: 10.26402/jpp.2020.2.10. Epub 2020 Aug 8.
Based on the previous studies, neurokinin B (NKB) participation in the modulation of prolactin secretion at the pituitary level can be assumed, but information concerning this topic is largely insufficient. Therefore, in the present study, we aimed: 1) to evaluate changes in the expression of NKB precursor (Tac3) and its receptor (Tacr3) genes as well as the content of NKB and TACR3 proteins in the porcine anterior pituitary throughout the estrous cycle (days 2 - 3, 9 - 10, 12 - 13, 15 - 16, 19 - 20); 2) to determine in vitro the influence of NKB on the expression of Prl, D2r and Trhr genes in the anterior pituitary cells (incubated for 4 h) as well as on prolactin secretion by these cells (incubated for 4 and 24 h) during chosen days of the estrous cycle (9 - 10, 15 - 16, 19 - 20). The experiments have shown alterations in the expression of Tacr3 mRNA and TACR3 protein content, but not in Tac3 mRNA and NKB protein. The treatment with NKB stimulated the expression of Prl (days 15 - 16), D2r (days 9 - 10) and Trhr (days 19 - 20) genes, but its potential to modulate prolactin secretion was observed only following 24-h incubation, specifically inhibition by NKB alone and stimulation by NKB with dopamine on days 19 - 10 of the cycle. These results indicate some implications of NKB in the modulation of prolactin secretion at the pituitary level in cyclic pigs, however further experiments are required to better clarify its role in this process.
基于先前的研究，可以假设神经激肽 B（NKB）参与了垂体水平催乳素分泌的调节，但关于这个主题的信息还远远不够。因此，在本研究中，我们旨在：1）评估发情周期（第 2-3、9-10、12-13、15-16、19-20 天）中猪前垂体中 NKB 前体（Tac3）及其受体（Tacr3）基因的表达变化以及 NKB 和 TACR3 蛋白的含量；2）确定 NKB 在发情周期（第 9-10、15-16、19-20 天）中对前垂体细胞中 Prl、D2r 和 Trhr 基因表达的体外影响以及对这些细胞中催乳素分泌的影响（孵育 4 和 24 小时）。实验表明 Tacr3 mRNA 和 TACR3 蛋白含量的表达发生了变化，但 Tac3 mRNA 和 NKB 蛋白没有变化。NKB 的处理刺激了 Prl（第 15-16 天）、D2r（第 9-10 天）和 Trhr（第 19-20 天）基因的表达，但仅在 24 小时孵育时观察到其调节催乳素分泌的潜力，具体为在周期第 19-10 天，NKB 单独抑制和 NKB 与多巴胺联合刺激。这些结果表明 NKB 在周期性猪垂体催乳素分泌的调节中具有一定的作用，但需要进一步的实验来更好地阐明其在该过程中的作用。
