Mantsch H H, Yang P W, Martin A, Cameron D G
Division of Chemistry, National Research Council of Canada, Ottawa.
Eur J Biochem. 1988 Dec 15;178(2):335-41. doi: 10.1111/j.1432-1033.1988.tb14455.x.
The physical state of the membrane lipids in the plasma membranes of intact, live Acholeplasma laidlawii B cells was probed by Fourier-transform infrared spectroscopy and compared with that in isolated membranes. Infrared spectra of live A. laidlawii B cells, enriched biosynthetically in the presence of avidin, with saturated deuterated and unsaturated non-deuterated fatty acids have been recorded at a variety of temperatures. The results indicate that within the temperature range of the gel to liquid-crystal phase transition, the live cells are able to keep the 'fluidity' of their plasma membranes at a considerably higher value compared to that in the isolated membranes at the same temperature. While this is a generally valid observation, the degree by which live and isolated membranes differ in their liquid-crystal-phase content at a given temperature depends on the nature of the exogenous fatty acid and the temperature of growth.
利用傅里叶变换红外光谱法探测完整活莱氏无胆甾原体B细胞膜中膜脂的物理状态，并与分离膜中的状态进行比较。在多种温度下记录了在抗生物素蛋白存在下通过生物合成富集了饱和氘代脂肪酸和不饱和非氘代脂肪酸的活莱氏无胆甾原体B细胞的红外光谱。结果表明，在凝胶到液晶相转变的温度范围内，与相同温度下的分离膜相比，活细胞能够将其质膜的“流动性”保持在相当高的值。虽然这是一个普遍有效的观察结果，但在给定温度下活膜和分离膜在液晶相含量上的差异程度取决于外源脂肪酸的性质和生长温度。
