Mason P, Strassman A, Maciewicz R
Pain Physiology Laboratory, Massachusetts General Hospital, Boston.
Exp Brain Res. 1988;73(1):1-7. doi: 10.1007/BF00279654.
To determine if PAG stimulation activates serotonin containing neurons in RM, intracellular techniques were used to identify raphe magnus (RM) cells that are excited by antinociceptive periaqueductal gray (PAG) stimulation in the cat. RM neurons that received a monosynaptic EPSP from PAG shock were intracellularly labeled with ethidium bromide. Intracellularly stained RM cells were large, medium and small sized neurons. To determine if these cells contain serotonin, sections containing intracellularly labeled RM neurons were processed for serotonin immunocytochemistry. None of the RM cells (N = 32) which were intracellularly stained with ethidium bromide contained immunoreactive serotonin. These results are evidence that non-serotonergic RM cells are activated by antinociceptive PAG stimulation; these cells may be important in the relay of PAG induced antinociception.
为了确定中脑导水管周围灰质（PAG）刺激是否激活中缝大核（RM）中含5-羟色胺的神经元，采用细胞内技术来识别猫中受抗伤害感受性中脑导水管周围灰质（PAG）刺激兴奋的中缝大核（RM）细胞。接受来自PAG电击单突触兴奋性突触后电位（EPSP）的RM神经元用溴化乙锭进行细胞内标记。细胞内染色的RM细胞有大、中、小三种尺寸的神经元。为了确定这些细胞是否含有5-羟色胺，对含有细胞内标记RM神经元的切片进行5-羟色胺免疫细胞化学处理。用溴化乙锭进行细胞内染色的RM细胞（N = 32）均未含有免疫反应性5-羟色胺。这些结果证明非5-羟色胺能RM细胞被抗伤害感受性PAG刺激激活；这些细胞可能在PAG诱导的抗伤害感受传导中起重要作用。
