通过一种新的双重标记方法对中枢去甲肾上腺素能和5-羟色胺能神经元进行细胞内鉴定。
Intracellular identification of central noradrenergic and serotonergic neurons by a new double labeling procedure.
作者信息
Aghajanian G K, Vandermaelen C P
出版信息
J Neurosci. 1982 Dec;2(12):1786-92. doi: 10.1523/JNEUROSCI.02-12-01786.1982.
Abstract
The purpose of this study was to ascertain the identity of presumed noradrenergic or serotonergic neurons recorded by single cell techniques in the mammalian brain. A double labeling method was developed in which intracellular injections of a red fluorescing dye (ethidium bromide) could be co-localized with the formaldehyde-induced green fluorescence of norepinephrine or yellow fluorescence of serotonin. By this method, neurons of the rat locus coeruleus that display a characteristic activation-inhibition response to noxious stimuli were confirmed to be noradrenergic; the slow, rhythmically firing neurons of the dorsal raphe nucleus were confirmed to be serotonergic.
摘要
本研究的目的是确定通过单细胞技术在哺乳动物大脑中记录的假定去甲肾上腺素能或5-羟色胺能神经元的身份。开发了一种双重标记方法，其中细胞内注射红色荧光染料（溴化乙锭）可与去甲肾上腺素的甲醛诱导绿色荧光或5-羟色胺的黄色荧光共定位。通过这种方法，对有害刺激表现出特征性激活-抑制反应的大鼠蓝斑核神经元被确认为去甲肾上腺素能神经元；中缝背核中缓慢、有节律放电的神经元被确认为5-羟色胺能神经元。
相似文献
1
Intracellular identification of central noradrenergic and serotonergic neurons by a new double labeling procedure.通过一种新的双重标记方法对中枢去甲肾上腺素能和5-羟色胺能神经元进行细胞内鉴定。
J Neurosci. 1982 Dec;2(12):1786-92. doi: 10.1523/JNEUROSCI.02-12-01786.1982.
2
Mutual interactions among cholinergic, noradrenergic and serotonergic neurons studied by ionophoresis of these transmitters in rat brainstem nuclei.通过在大鼠脑干核团中对这些递质进行离子电泳研究胆碱能、去甲肾上腺素能和5-羟色胺能神经元之间的相互作用。
Neuroscience. 1993 Aug;55(4):1117-26. doi: 10.1016/0306-4522(93)90325-a.
3
Effects of a potential antipsychotic, BMY 14802, on firing of central serotonergic and noradrenergic neurons in rats.
Eur J Pharmacol. 1990 Apr 25;179(3):357-66. doi: 10.1016/0014-2999(90)90176-7.
4
Distribution and origin of noradrenergic and serotonergic fibers in the cochlear nucleus and inferior colliculus of the rat.大鼠耳蜗核和下丘中去甲肾上腺素能和5-羟色胺能纤维的分布及起源
Brain Res. 1991 Aug 23;557(1-2):190-201. doi: 10.1016/0006-8993(91)90134-h.
5
Sensory responsiveness of "broad-spike" neurons in the laterodorsal tegmental nucleus, locus coeruleus and dorsal raphe of awake rats: implications for cholinergic and monoaminergic neuron-specific responses.清醒大鼠脑桥背外侧被盖核、蓝斑和中缝背核中“宽峰”神经元的感觉反应性：对胆碱能和单胺能神经元特异性反应的影响
Neuroscience. 1994 Dec;63(4):1021-31. doi: 10.1016/0306-4522(94)90569-x.
6
Potential noradrenergic regulation of serotonergic neurons in the median raphe nucleus.中缝正中核中5-羟色胺能神经元的潜在去甲肾上腺素能调节。
Exp Brain Res. 1979 Jan 2;34(1):177-82. doi: 10.1007/BF00238350.
7
Serotonergic innervation of the locus coeruleus from the dorsal raphe and its action on responses to noxious stimuli.来自中缝背核的蓝斑5-羟色胺能神经支配及其对伤害性刺激反应的作用。
J Physiol. 1979 Jan;286:401-15. doi: 10.1113/jphysiol.1979.sp012628.
8
Audiogenic seizures in rats: relation to noradrenergic neurons of the locus coeruleus.
Acta Physiol Pol. 1978 Sep-Oct;29(5):409-12.
9
Effects of clonidine, piperoxane and locus coeruleus lesion on the serotonergic and dopaminergic systems in raphe and caudate nucleus.可乐定、哌氧环烷及蓝斑损毁对中缝核和尾状核中5-羟色胺能及多巴胺能系统的影响。
Biochem Pharmacol. 1984 Nov 1;33(21):3399-404. doi: 10.1016/0006-2952(84)90111-4.
10
Aging effects on monoamines in rat medial vestibular and cochlear nuclei.衰老对大鼠内侧前庭核和耳蜗核中单胺类物质的影响。
Hear Res. 1996 Oct;100(1-2):150-6. doi: 10.1016/0378-5955(96)00116-5.
引用本文的文献
1
Dorsal and median raphe neuronal firing dynamics characterized by nonlinear measures.背侧和中缝核神经元放电动力学的非线性测度特征。
PLoS Comput Biol. 2024 May 28;20(5):e1012111. doi: 10.1371/journal.pcbi.1012111. eCollection 2024 May.
2
Selective Enhancement of REM Sleep in Male Rats through Activation of Melatonin MT Receptors Located in the Locus Ceruleus Norepinephrine Neurons.通过激活位于蓝斑去甲肾上腺素能神经元中的褪黑素MT受体选择性增强雄性大鼠的快速眼动睡眠。
J Neurosci. 2024 Jul 17;44(29):e0914232024. doi: 10.1523/JNEUROSCI.0914-23.2024.
3
Elevated Serotonin in Mouse Spinal Dorsal Horn Is Pronociceptive.小鼠脊髓背角中升高的 5-羟色胺具有致痛作用。
eNeuro. 2023 Dec 4;10(12). doi: 10.1523/ENEURO.0293-23.2023. Print 2023 Dec.
4
Organic cation transporter 2 contributes to SSRI antidepressant efficacy by controlling tryptophan availability in the brain.有机阳离子转运体 2 通过控制大脑中色氨酸的可用性来影响 SSRI 类抗抑郁药的疗效。
Transl Psychiatry. 2023 Sep 29;13(1):302. doi: 10.1038/s41398-023-02596-y.
5
Selective Modulation of K Channel Kv7.4 Significantly Affects the Excitability of DRN 5-HT Neurons.钾通道Kv7.4的选择性调节对中缝背核5-羟色胺能神经元的兴奋性有显著影响。
Front Cell Neurosci. 2017 Dec 14;11:405. doi: 10.3389/fncel.2017.00405. eCollection 2017.
6
Firing Properties of Genetically Identified Dorsal Raphe Serotonergic Neurons in Brain Slices.脑片中基因鉴定的中缝背侧5-羟色胺能神经元的放电特性
Front Cell Neurosci. 2016 Aug 3;10:195. doi: 10.3389/fncel.2016.00195. eCollection 2016.
7
Alterations in alpha5* nicotinic acetylcholine receptors result in midbrain- and hippocampus-dependent behavioural and neural impairments.α5*烟碱型乙酰胆碱受体的改变会导致中脑和海马体依赖的行为和神经损伤。
Psychopharmacology (Berl). 2016 Sep;233(18):3297-314. doi: 10.1007/s00213-016-4362-2. Epub 2016 Jul 6.
8
BDNF overexpression in mouse hippocampal astrocytes promotes local neurogenesis and elicits anxiolytic-like activities.BDNF 在小鼠海马星形胶质细胞中的过表达促进局部神经发生并引发抗焦虑样活性。
Transl Psychiatry. 2013 Apr 30;3(4):e253. doi: 10.1038/tp.2013.30.
9
Evidence that adrenergic ventrolateral medullary cells are activated whereas precerebellar lateral reticular nucleus neurons are suppressed during REM sleep.证据表明，去甲肾上腺素能腹外侧延髓细胞在 REM 睡眠期间被激活，而小脑前外侧网状核神经元被抑制。
PLoS One. 2013 Apr 22;8(4):e62410. doi: 10.1371/journal.pone.0062410. Print 2013.
10
A subpopulation of serotonergic neurons that do not express the 5-HT1A autoreceptor.不表达 5-HT1A 自身受体的血清素能神经元亚群。
ACS Chem Neurosci. 2013 Jan 16;4(1):89-95. doi: 10.1021/cn300157s. Epub 2012 Nov 7.
Zotero 插件