Aghajanian G K, Vandermaelen C P
J Neurosci. 1982 Dec;2(12):1786-92. doi: 10.1523/JNEUROSCI.02-12-01786.1982.
The purpose of this study was to ascertain the identity of presumed noradrenergic or serotonergic neurons recorded by single cell techniques in the mammalian brain. A double labeling method was developed in which intracellular injections of a red fluorescing dye (ethidium bromide) could be co-localized with the formaldehyde-induced green fluorescence of norepinephrine or yellow fluorescence of serotonin. By this method, neurons of the rat locus coeruleus that display a characteristic activation-inhibition response to noxious stimuli were confirmed to be noradrenergic; the slow, rhythmically firing neurons of the dorsal raphe nucleus were confirmed to be serotonergic.
本研究的目的是确定通过单细胞技术在哺乳动物大脑中记录的假定去甲肾上腺素能或5-羟色胺能神经元的身份。开发了一种双重标记方法，其中细胞内注射红色荧光染料（溴化乙锭）可与去甲肾上腺素的甲醛诱导绿色荧光或5-羟色胺的黄色荧光共定位。通过这种方法，对有害刺激表现出特征性激活-抑制反应的大鼠蓝斑核神经元被确认为去甲肾上腺素能神经元；中缝背核中缓慢、有节律放电的神经元被确认为5-羟色胺能神经元。
