University of Technology Sydney (UTS), Centre for Forensic Science, Sydney, NSW, Australia.
University of Technology Sydney (UTS), Centre for Forensic Science, Sydney, NSW, Australia.
Forensic Sci Int. 2020 Apr;309:110185. doi: 10.1016/j.forsciint.2020.110185. Epub 2020 Feb 7.
Fingermarks and DNA are valuable traces in forensic investigations potentially allowing for the identification of the source of the trace or highlighting a link between a touched object and an individual. These traces are often latent and need to be detected before recovery. While a number of validated methods exist for fingermark detection, no routine method is in place for the detection of DNA. This study investigates the use of pdimethylaminobenzaldehyde (DMAB) in conjunction with indanedione zinc (IND-Zn) for the detection of latent cellular material and fingermarks on paper. The aim of this proof-of-concept study is to determine the successfulness of this reagent (DMAB-IND) in the detection of the respective traces and observe the impact it has on the resulting DNA profile. It was found that latent fingermarks and the associated cells could be visualised following treatment with the reagent. Samples treated with DMAB-IND showed a significantly higher percentage of alleles called compared to IND-Zn-treated and untreated samples due to the targeted recovery of cells. However, the reagent appears to degrade DNA at a rapid rate, requiring the treated samples to be processed for DNA on the day of treatment.
指纹和 DNA 是法医学调查中非常有价值的痕迹，它们有可能确定痕迹的来源，或者突出被触碰物体与个体之间的联系。这些痕迹通常是潜伏的，需要在恢复之前进行检测。虽然已经存在许多验证过的指纹检测方法，但对于 DNA 的检测还没有常规方法。本研究调查了二甲氨基苯甲醛 (DMAB) 与茚满二酮锌 (IND-Zn) 联合用于检测纸张上潜伏细胞物质和指纹的用途。本概念验证研究的目的是确定该试剂 (DMAB-IND) 在检测各自痕迹时的成功程度，并观察它对最终 DNA 图谱的影响。结果发现，在用该试剂处理后，可以观察到潜伏指纹和相关细胞。与 IND-Zn 处理和未处理的样本相比，用 DMAB-IND 处理的样本中被称为等位基因的数量明显更高，这是由于细胞的靶向回收。然而，该试剂似乎会迅速降解 DNA，因此需要在处理当天对处理过的样本进行 DNA 处理。
