Loganathan Gopalakrishnan, Balamurugan Appakalai N, Venugopal Subhashree
Clinical Islet Cell Laboratory, Cardiovascular Innovation Institute, Department of Surgery, University of Louisville, Louisville, KY, USA; School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India.
Clinical Islet Cell Laboratory, Cardiovascular Innovation Institute, Department of Surgery, University of Louisville, Louisville, KY, USA.
Diabetes Metab Syndr. 2020 Mar-Apr;14(2):159-166. doi: 10.1016/j.dsx.2020.01.010. Epub 2020 Jan 30.
Successful clinical human allo or auto-islet transplantation requires the recovery of a sufficient number of functional islets from either brain-dead or chronic pancreatitis pancreases respectively.
In the last two decades (2000-2019), significant progress has been made in improving the human islet isolation procedures and in standardizing the use of different tissue dissociation enzyme (TDE; a mixture of collagenase and protease enzymes) blends to recover higher islet yields.
This review presents information focusing on properties and role of TDE blends during the islet isolation process, particularly emphasizing on the current developments, associated challenges and future perspectives within the field.
成功的临床人类同种异体或自体胰岛移植分别需要从脑死亡或慢性胰腺炎胰腺中获取足够数量的功能正常的胰岛。
在过去二十年（2000 - 2019年）中，在改进人类胰岛分离程序以及标准化使用不同组织解离酶（TDE；胶原酶和蛋白酶的混合物）混合物以提高胰岛产量方面取得了显著进展。
本综述介绍了有关TDE混合物在胰岛分离过程中的特性和作用的信息，特别强调了该领域的当前发展、相关挑战和未来前景。
