用于胰岛分离的人胰腺组织解离酶：进展与临床前景。
Human pancreatic tissue dissociation enzymes for islet isolation: Advances and clinical perspectives.
作者信息
Loganathan Gopalakrishnan, Balamurugan Appakalai N, Venugopal Subhashree
机构信息
Clinical Islet Cell Laboratory, Cardiovascular Innovation Institute, Department of Surgery, University of Louisville, Louisville, KY, USA; School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India.
Clinical Islet Cell Laboratory, Cardiovascular Innovation Institute, Department of Surgery, University of Louisville, Louisville, KY, USA.
出版信息
Diabetes Metab Syndr. 2020 Mar-Apr;14(2):159-166. doi: 10.1016/j.dsx.2020.01.010. Epub 2020 Jan 30.
BACKGROUND AND AIMS
Successful clinical human allo or auto-islet transplantation requires the recovery of a sufficient number of functional islets from either brain-dead or chronic pancreatitis pancreases respectively.
METHODS
In the last two decades (2000-2019), significant progress has been made in improving the human islet isolation procedures and in standardizing the use of different tissue dissociation enzyme (TDE; a mixture of collagenase and protease enzymes) blends to recover higher islet yields.
RESULTS AND CONCLUSIONS
This review presents information focusing on properties and role of TDE blends during the islet isolation process, particularly emphasizing on the current developments, associated challenges and future perspectives within the field.
背景与目的
成功的临床人类同种异体或自体胰岛移植分别需要从脑死亡或慢性胰腺炎胰腺中获取足够数量的功能正常的胰岛。
方法
在过去二十年（2000 - 2019年）中，在改进人类胰岛分离程序以及标准化使用不同组织解离酶（TDE；胶原酶和蛋白酶的混合物）混合物以提高胰岛产量方面取得了显著进展。
结果与结论
本综述介绍了有关TDE混合物在胰岛分离过程中的特性和作用的信息，特别强调了该领域的当前发展、相关挑战和未来前景。
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