De Haan L D, Werre J M, Ruben A M, Huls A H, de Gier J, Staal G E
Department of Medicine, Municipal Hospital, Arnhem, The Netherlands.
Eur J Haematol. 1988 Nov;41(5):482-8. doi: 10.1111/j.1600-0609.1988.tb00231.x.
Whole blood from splenectomized subjects (n = 8) contained a substantial percentage of vacuolated ('pitted') red cells (34.7 +/- 8.3%), while blood from controls revealed none. The percentage of haemoglobin A1 (HbA1) had increased significantly compared with controls (p less than 0.01). Fractionation on cell density revealed that the number of 'pitted' cells and the HbA1 percentage were associated with increased cell density. Fractionation on cell volume showed that 'pitted' cells are equally distributed in fractions with varying mean cell volume (MCV) and that decreasing MCV was associated with a linear rise of HbA1. It appeared that, shortly after splenectomy (n = 4), 'pits' develop early in cell life and that also older cells, after previous splenic contact, are capable of pit formation. A positive correlation found between the number of 'pitted' cells and the percentage of HbA1 might point to an impaired release of HbA1, manifest in the presence of 'pits'. The increased percentage of HbA1 in whole blood from splenectomized subjects may thus be explained.
脾切除患者（n = 8）的全血中含有相当比例的空泡化（“去核”）红细胞（34.7±8.3%），而对照组血液中未发现此类细胞。与对照组相比，血红蛋白A1（HbA1）的百分比显著增加（p<0.01）。按细胞密度进行分级分离显示，“去核”细胞数量和HbA1百分比与细胞密度增加相关。按细胞体积进行分级分离表明，“去核”细胞均匀分布于平均细胞体积（MCV）不同的分级中，且MCV降低与HbA1呈线性升高相关。似乎在脾切除术后不久（n = 4），“空泡”在细胞生命早期形成，并且之前接触过脾脏的较老细胞也能够形成空泡。“去核”细胞数量与HbA1百分比之间的正相关可能表明HbA1释放受损，表现为空泡的存在。因此，可以解释脾切除患者全血中HbA1百分比增加的现象。
