Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Methods Mol Biol. 2020;2105:251-260. doi: 10.1007/978-1-0716-0243-0_16.
Using near-infrared fluorophore Alexa Fluor 680 labeled peptide nucleic acids (PNAs) the biodistribution of such antisense agents can be analyzed in real time in live mice using in vivo imaging. Using the fluorescence intensity emitted from the mouse at different time points following administration, the systemic distribution and organ accumulation of PNA can be tracked. In addition, an estimation of the body half-life of the compound can be obtained by the change in fluorescence intensity over time. With this technique, the distribution of compounds can be monitored real time, while reducing the number of animals and amount of compounds required.
使用近红外荧光染料 Alexa Fluor 680 标记的肽核酸 (PNA)，可以使用活体动物体内成像技术实时分析这些反义试剂在活体小鼠中的分布情况。通过在给药后不同时间点从小鼠发出的荧光强度，可以跟踪 PNA 的全身分布和器官积累。此外，通过随时间变化的荧光强度，可以估计化合物的体半衰期。通过该技术，可以实时监测化合物的分布，同时减少所需动物数量和化合物用量。
