Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Methods Mol Biol. 2020;2126:33-43. doi: 10.1007/978-1-0716-0364-2_4.
Cells can be easily and noninvasively tracked in the body by labeling them with a lipophilic, near-infrared dye and using a live fluorescence imaging system to image the position of the dye in the body. Near-infrared dyes provide several advantages, primarily that tissue is mostly highly transparent to near-infrared light, resulting in clearer and more accurate images. Briefly, cells are labeled with a near-infrared dye such as DiR and injected into a disease model. The model is then imaged using the live fluorescence imaging system on an hourly and/or daily basis to track cell migration and final location. The relative number of cells that migrate to the desired location can be measured by measuring the fluorescent intensity at the location versus elsewhere in the body. This paper describes a method for using DiR dye to label and track C17.2 neural progenitor cells to a murine model of mammary carcinoma.
细胞可以通过用亲脂性近红外染料进行标记，并使用活体荧光成像系统来对体内染料的位置进行成像，从而在体内轻松且无创地进行追踪。近红外染料具有几个优点，主要是组织对近红外光的高透明性，从而产生更清晰、更准确的图像。简而言之，用近红外染料（如 DiR）对细胞进行标记，然后将其注入疾病模型。然后使用活体荧光成像系统每隔一小时和/或每天对模型进行成像，以追踪细胞的迁移和最终位置。通过测量该位置与体内其他位置的荧光强度，可以测量迁移到所需位置的细胞的相对数量。本文描述了一种使用 DiR 染料标记和追踪 C17.2 神经祖细胞到乳腺癌鼠模型的方法。
