[Cytochrome P-450-dependent mechanisms of the biosynthesis of protein-conjugated benzo(a)pyrene antigens and their role in the development of a specific immune response to this carcinogen].

There was studied the possibility of covalent binding to proteins of a carcinogenic compound benzo(a)pyrene in the system of cytochrome P-450 of the liver and the possibility of the development of the immune reaction to administration of the conjugated antigens obtained in such a way to animals. It was shown that under experimental conditions modelling the processes of microsomal oxidation of benzo(a)pyrene in the organism there occurs irreversible binding of 14C-benzo(a)pyrene both to microsomes and the added albumin. Immunization of rabbits by conjugates benzo(a)pyrene-albumin and benzo(a)pyrene-microsomes leads to the development of the immune response to the original carcinogen. The antibodies and lymphocytes specifically binding 14C-benzo(a)pyrene appear in the blood.