Nakamura S, Demura R, Komatsu H, Suzuki T, Jibiki K, Odagiri E, Demura H
Radioassay Center, Tokyo Women's Medical College, Japan.
Nihon Naibunpi Gakkai Zasshi. 1988 Oct 20;64(10):1102-14. doi: 10.1507/endocrine1927.64.10_1102.
To investigate the significance of changes in ovarian inhibin in gonadal dysfunction with persistent estrus, female rats with persistent estrus were studied as animal model for polycystic ovaries (PCO). Newborn female Wistar rats treated with testosterone propionate s. c. (TP) and female rats raised under continuous light (LL) started at 9 weeks of age entered into continuous estrus 5 and 2 or 3 weeks after the treatment, respectively. Ovaries in both groups had many polycystic follicles Normal adult female rats with 4 day cycles were served as control. Ovarian inhibin content was measured by FSH suppressing activity of charcoal-treated ovarian homogenates using a rat anterior pituitary cell culture system. Blood samples were obtained at 11:00 a. m. and plasma levels of LH, FSH, PRL, estradiol (E2), progesterone (P) and testosterone were measured by RIA. Comparisons were made statistically between each experimental group and normal proestrus (PE). Both LH and FSH were similar to normal PE in TP LL, suggesting a lack of LH and FSH surges probably through hypothalamic dysfunction in TP and LL. Plasma PRL levels were variably elevated in both groups. Ovarian inhibin contents were comparably high to PE in both groups with parallel increases in plasma E2 and P. Plasma testosterone was elevated only in TP. In conclusion, female rats with persistent estrus induced by TP or LL exhibited polycystic ovarian changes similar to PCO. Increased inhibin content in TP and LL may reflect persistent follicular activity.
为研究卵巢抑制素变化在持续性发情所致性腺功能障碍中的意义，以持续性发情的雌性大鼠作为多囊卵巢（PCO）动物模型进行研究。皮下注射丙酸睾酮（TP）处理的新生雌性Wistar大鼠以及9周龄起饲养于持续光照（LL）条件下的雌性大鼠，分别在处理后5周和2或3周进入持续性发情期。两组大鼠的卵巢均有许多多囊卵泡。以4天为周期的正常成年雌性大鼠作为对照。采用大鼠垂体前叶细胞培养系统，通过测定经活性炭处理的卵巢匀浆抑制FSH的活性来检测卵巢抑制素含量。于上午11:00采集血样，采用放射免疫分析法测定血浆中LH、FSH、PRL、雌二醇（E2）、孕酮（P）和睾酮水平。对各实验组与正常动情前期（PE）进行统计学比较。在TP和LL组中，LH和FSH水平均与正常PE相似，提示可能由于TP和LL组下丘脑功能障碍导致LH和FSH峰缺失。两组血浆PRL水平均有不同程度升高。两组卵巢抑制素含量均与PE相当，且血浆E2和P水平呈平行升高。仅TP组血浆睾酮水平升高。总之，由TP或LL诱导产生持续性发情的雌性大鼠表现出与PCO相似的多囊卵巢变化。TP和LL组抑制素含量增加可能反映了卵泡的持续活动。
