Sirotkin Alexander V, Tarko Adam, Kotwica Jan, Alrezaki Abdulkarem, Harrath Abdel Halim
Department of Zoology and Anthropology, Constantine the Philosopher University, Nitra, Slovakia.
Department of Zoology and Anthropology, Constantine the Philosopher University, Nitra, Slovakia.
Reprod Biol. 2020 Jun;20(2):254-258. doi: 10.1016/j.repbio.2020.02.003. Epub 2020 Feb 20.
We examined the effects of metabolic hormones leptin and ghrelin, and the oil-related environmental contaminants toluene and xylene on the release of ovarian hormones by gravid and non-gravid cats, as well as the functional interrelationships between metabolic hormones and contaminants. Ovarian fragments of non-gravid cats were cultured with and without leptin and toluene. Next, ovarian fragments of either non-gravid or gravid animals were cultured with and without ghrelin and xylene. Oxytocin (OT) and prostaglandin F (PGF) release was measured using ELISA. We confirm ovarian OT and PGF production by feline ovary, demonstrate the involvement of leptin and ghrelin in controlling OT and PGF release, show the direct influence of toluene and xylene on feline ovarian secretory activity, indicate the ability of leptin and ghrelin to mimic and promote the main contaminant effects, demonstrate that oil-related contaminants can prevent and even invert the effects of leptin and ghrelin on the ovary, and suggest the gravidity-associated changes in ability of ghrelin to promote xylene action on PGF (but not to OT), but not in basic ovarian OT and PGF release and their response to ghrelin or xylene.
我们研究了代谢激素瘦素和胃饥饿素，以及与石油相关的环境污染物甲苯和二甲苯对妊娠和未妊娠猫卵巢激素释放的影响，以及代谢激素与污染物之间的功能相互关系。将未妊娠猫的卵巢组织块分别在添加和不添加瘦素及甲苯的情况下进行培养。接下来，将未妊娠或妊娠动物的卵巢组织块分别在添加和不添加胃饥饿素及二甲苯的情况下进行培养。使用酶联免疫吸附测定法（ELISA）测量催产素（OT）和前列腺素F（PGF）的释放。我们证实了猫卵巢可产生OT和PGF，证明了瘦素和胃饥饿素参与控制OT和PGF的释放，显示了甲苯和二甲苯对猫卵巢分泌活动的直接影响，表明瘦素和胃饥饿素能够模拟并促进主要污染物的作用，证明与石油相关的污染物可预防甚至逆转瘦素和胃饥饿素对卵巢的影响，并表明妊娠相关变化影响胃饥饿素促进二甲苯对PGF（而非OT）作用的能力，但不影响卵巢基础OT和PGF的释放及其对胃饥饿素或二甲苯的反应。
