Jiang Wei, Liu Ping, Zhang Jianlei, Yang Wenjie
1Department of Infectious Diseases, Tianjin First Center Hospital, No. 24 Fukang Road, Nankai District, Tianjin, 300192 China.
2Laboratory of Microbiology of Tianjin First Center Hospital, Tianjin, China.
Indian J Microbiol. 2020 Mar;60(1):62-69. doi: 10.1007/s12088-019-00847-5. Epub 2019 Dec 13.
is a common opportunistic pathogen that can cause serious infection by blood transmission. enters the blood circulation and adheres to the endothelial cells of the vascular wall. However, the detailed mechanism of the effect of on the endothelial cells remains unclear. In this study, the microarray expression profile of human umbilical vein endothelial cells exposed to was analyzed. The 191 up-regulated genes were enriched in TNF, T cell receptor, and NF-kappa B signaling pathways. The 71 down-regulated genes were enriched in pyruvate metabolic, purine nucleotide metabolic, purine nucleotide biosynthetic, and humoral immune response processes. Gene set enrichment analysis showed that apoptosis, oxidative phosphorylation, IL6/JAK/STAT3 signaling pathways were enriched. Moreover, two hub genes with a high degree of connectivity, namely, MYC and IL6, were selected. Molecular screening of traditional Chinese medicine libraries was performed on the basis of the structure of MYC protein. The okanin had the highest docking score. MYC might be used as molecular targets for treatment. In addition, okanin may inhibit the infection of . Thus, MYC can be subjected to further research.
是一种常见的机会性病原体，可通过血液传播引起严重感染。进入血液循环并粘附于血管壁的内皮细胞。然而，对内皮细胞作用的详细机制仍不清楚。在本研究中，分析了暴露于的人脐静脉内皮细胞的微阵列表达谱。191个上调基因富集于TNF、T细胞受体和NF-κB信号通路。71个下调基因富集于丙酮酸代谢、嘌呤核苷酸代谢、嘌呤核苷酸生物合成和体液免疫应答过程。基因集富集分析表明，凋亡、氧化磷酸化、IL6/JAK/STAT3信号通路被富集。此外，选择了两个具有高度连通性的枢纽基因，即MYC和IL6。基于MYC蛋白的结构对中药文库进行分子筛选。奥卡宁具有最高的对接分数。MYC可能用作治疗的分子靶点。此外，奥卡宁可能抑制的感染。因此，MYC可进行进一步研究。
