Maoloni Antonietta, Milanović Vesna, Cardinali Federica, Mangia Nicoletta P, Murgia Marco A, Garofalo Cristiana, Clementi Francesca, Osimani Andrea, Aquilanti Lucia
1Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
2Dipartimento di Agraria, Università degli Studi di Sassari, Viale Italia 39, 07100 Sassari, Italy.
Indian J Microbiol. 2020 Mar;60(1):119-123. doi: 10.1007/s12088-019-00838-6. Epub 2019 Nov 5.
is the sole variety of fermented milk originating in Italy. Despite the long history of consumption, still represents an undisclosed source of microbial diversity. The present study was aimed to get an insight into the bacterial and fungal diversity of samples collected from two artisan producers located in Sardinia. To this end 3 batches of were collected from each producer and subjected to Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) analyses. was produced with sheep milk in accordance with the local tradition. Regarding the bacterial population, a low biodiversity emerged. In more detail, the sole species subsp. was detected in all the samples, irrespective of the producer or the batch. A more ample microbial diversity was highlighted for the fungal population that included closest relatives to , and . Based on the results, the detected bacterial and fungal species generally clustered in accordance with the producer, irrespective of the batch considered. It is noteworthy that, revealed several microbiological similarities with kefir beverage, thus suggesting, by analogy, potential health benefits related to its consumption. More research is needed to better clarify the microbiota composition of by using more powerful metagenomic techniques.
是源自意大利的唯一一种发酵乳。尽管其消费历史悠久，但仍是微生物多样性的一个未被揭示的来源。本研究旨在深入了解从撒丁岛的两个手工生产者处采集的样品中的细菌和真菌多样性。为此，从每个生产者处收集了3批样品，并进行聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析。是按照当地传统用羊奶生产的。关于细菌种群，出现了低生物多样性。更详细地说，在所有样品中都检测到了唯一的亚种。对于真菌种群，突出显示了更丰富的微生物多样性，其中包括与、和关系最密切的亲属。基于这些结果，检测到的细菌和真菌物种通常根据生产者聚类，而不考虑所考虑的批次。值得注意的是，与开菲尔饮料显示出若干微生物学相似性，因此类推表明与其消费相关的潜在健康益处。需要更多研究以通过使用更强大的宏基因组技术更好地阐明的微生物群组成。
