印度与象腿芋相关植原体的特征及遗传多样性
Characterization and genetic diversity of phytoplasmas associated with elephant foot yam in India.
作者信息
Rao G P, Reddy Madem Gurivi, Priya Madhu, Bahadur Amar, Dubey Durgesh K
机构信息
Division of Plant Pathology, Indian Agriculture Research Institute, Pusa Campus, New Delhi, 110012 India.
College of Agriculture, Lembucherra, West Tripura 799210 India.
出版信息
3 Biotech. 2020 Mar;10(3):83. doi: 10.1007/s13205-020-2069-x. Epub 2020 Feb 3.
During the growing season of 2015 and 2016, leaf yellowing, stunting, and declining symptoms were observed on elephant foot yam in three states of India.The 1.3 kb 16S rDNA fragments were amplified from genomic DNA extracted from all the symptomatic elephant foot yam samples in nested PCR assays using primer pairs, P1/P7 followed by 3F/3R. Pair wise sequence comparison, virtual RFLP and phylogenetic analysis of 16S rRNA gene sequences confirmed association of ' Phytoplasma trifolii' (16SrVI-D) and ' Phytoplasma oryzae' (16SrXI-B) related strains in Uttar Pradesh, Uttarakhand and Tripura states, respectively. To our knowledge, this is the first report of association of '. ' and '. ' related strains in elephant foot yam in world. In the present study, we also reported showing witches' broom as a natural weed host for '. ' phytoplasma in Gorakhpur and Kushinagar districts of Uttar Pradesh state, India.
在2015年和2016年的生长季节，印度三个邦的象腿芋上观察到叶片发黄、发育不良和衰退症状。使用引物对P1/P7随后3F/3R，通过巢式PCR分析从所有有症状的象腿芋样本提取的基因组DNA中扩增出1.3 kb的16S rDNA片段。16S rRNA基因序列的成对序列比较、虚拟RFLP和系统发育分析分别证实了北方邦、北阿坎德邦和特里普拉邦存在与“三叶草植原体”（16SrVI-D）和“稻植原体”（16SrXI-B）相关的菌株。据我们所知，这是世界上关于象腿芋中与“.”和“.”相关菌株关联的首次报道。在本研究中，我们还报道了在印度北方邦戈勒克布尔和库什纳加尔地区，显示出扫帚状丛枝病的野生杂草是“.”植原体的天然寄主。
Zotero 插件