Katsafadou Angeliki I, Tsangaris George Th, Vasileiou Natalia G C, Ioannidi Katerina S, Anagnostopoulos Athanasios K, Billinis Charalambos, Fragkou Ilektra A, Papadopoulos Elias, Mavrogianni Vasia S, Michael Charalambia K, Addis M Filippa, Fthenakis George C
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece.
Proteomics Research Unit, Biomedical Research Foundation of Academy of Athens, 11527 Athens, Greece.
Data Brief. 2020 Feb 8;29:105259. doi: 10.1016/j.dib.2020.105259. eCollection 2020 Apr.
Bacteriological, cytological and proteomics data have been obtained from ewes in two experiments, after intramammary challenge with or . Animals were sampled before and sequentially after challenge. Conventional techniques were employed for bacterial isolation and somatic cell counting in milk samples; milk whey samples were subjected to proteomics evaluation by using two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. There was a correlation between leucocyte content and cathelicidin-1 spot densities in milk samples, although the protein was detected in milk earlier than the increase in leucocyte content. There was also a significant association between presence of mastitis in a mammary gland and detection of cathelicidin-1 in the respective milk sample; the degree of association was greater during the first 24 h post-inoculation. The data are further discussed in the research article "Detection of cathelicidin-1 in the milk as an early indicator of mastitis in ewes" [1].
在两项实验中，对母羊进行乳房内接种或后，获取了细菌学、细胞学和蛋白质组学数据。在接种前后对动物进行了采样。采用常规技术对牛奶样本进行细菌分离和体细胞计数；利用二维凝胶电泳和基质辅助激光解吸电离飞行时间质谱对乳清样本进行蛋白质组学评估。尽管在牛奶中检测到该蛋白质的时间早于白细胞含量增加的时间，但牛奶样本中的白细胞含量与cathelicidin-1斑点密度之间存在相关性。乳腺中乳腺炎的存在与相应牛奶样本中cathelicidin-1的检测之间也存在显著关联；在接种后的最初24小时内，关联程度更大。研究文章《检测牛奶中的cathelicidin-1作为母羊乳腺炎的早期指标》[1]中对这些数据进行了进一步讨论。
