Manufacturing Division, Yamasa Corporation, Chiba, Japan.
Biosci Biotechnol Biochem. 2020 Jun;84(6):1291-1298. doi: 10.1080/09168451.2020.1732187. Epub 2020 Feb 24.
Some wild impair the quality of soy sauce through the generation of unpleasant odors induced by the formation of flor. Flor formation in depends on the expression of the gene, which is a homolog of the gene that encodes a cell surface protein in expression in is regulated by multiple pathways. To investigate the regulation of expression in , we created 13 gene knockout mutants (, and ) related to those pathways and examined whether these mutants form flor. Unexpectedly, knockout mutant could only form a very weak flor due to decreased expression, suggesting that acts as a potential activator of flor formation through expression. This result is in contrast to , which acts as a repressor of expression.
一些野生酵母通过形成花醭产生不愉快的气味来降低酱油的质量。花醭的形成取决于 floc 形成基因的表达,该基因是编码 细胞表面蛋白的 基因的同源物, 在 中的表达受多个途径调控。为了研究 在 中的表达调控,我们创建了与这些途径相关的 13 个基因敲除突变体( 和 ),并检查这些突变体是否形成花醭。出乎意料的是, 敲除突变体由于 表达减少只能形成非常微弱的花醭,这表明 通过 表达作为花醭形成的潜在激活剂发挥作用。这一结果与 相反, 作为 表达的抑制剂。